Chapter 19, Problem 2
Write a short essay or sketch a diagram that provides an overview of how recombinant DNA techniques help geneticists study genes.
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Write a short essay that summarizes the impacts that genomic applications are having on society and discuss which of the ethical issues presented by these applications is the most daunting to society.
Why are most recombinant human proteins produced in animal or plant hosts instead of bacterial host cells?
What roles do restriction enzymes, vectors, and host cells play in recombinant DNA studies? What role does DNA ligase perform in a DNA cloning experiment? How does the action of DNA ligase differ from the function of restriction enzymes?
The human insulin gene contains a number of sequences that are removed in the processing of the mRNA transcript. In spite of the fact that bacterial cells cannot excise these sequences from mRNA transcripts, explain how a gene like this can be cloned into a bacterial cell and produce insulin.