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Ch. 20 - Recombinant DNA Technology
All textbooksKlug 12th EditionCh. 20 - Recombinant DNA TechnologyProblem 3
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Chapter 19, Problem 3

What roles do restriction enzymes, vectors, and host cells play in recombinant DNA studies? What role does DNA ligase perform in a DNA cloning experiment? How does the action of DNA ligase differ from the function of restriction enzymes?

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Hi, everybody. Let's take a look at this practice problem together. Blank is the molecule glue that is used in the cloning experiment to join two fragments of D N A to seal the gaps between them. A quick glance at the answer options. And we can see they all end in A S E. That means our molecule glue is an enzyme. Our answer will be an enzyme And it's an enzyme that Catalyze is a bond and joins two fragments of DNA. So let's discuss our answer options. We've got a restriction endo nuclear's now recall that a restriction endo nuclear's recognizes certain DNA sequences called restriction sites. And that cleaves at these sites. It's common for them to be used in recombinant DNA technology. And therefore, this is the opposite of what we are looking for. So A is not the correct answer. Then we've got be topo Y summaries. Recall that topo I summaries breaks and rejoins D N A to fix super coiling. Now super coiling can occur as a result of DNA replication and transcription. Therefore, since this breaks and rejoins D N A option B is not the correct answer. Then we have options. See DNA, like gaze and the prefix liga means to bind. So this is an enzyme that binds DNA fragments together. It capitalizes the formation of a phosphor diaspora bond and allows the nucleotides to be connected to each other. Therefore, c is the correct answer. Alright, everyone, I hope you found this helpful and I'll see you soon for the next practice problem.
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One of the major causes of sickness, death, and economic loss in the cattle industry is Mannheimia haemolytica, which causes bovine pasteurellosis, or shipping fever. Noninvasive delivery of a vaccine using transgenic plants expressing immunogens would reduce labor costs and trauma to livestock. An early step toward developing an edible vaccine is to determine whether an injected version of an antigen (usually a derivative of the pathogen) is capable of stimulating the development of antibodies in a test organism. The following table assesses the ability of a transgenic portion of a toxin (Lkt) of M. haemolytica to stimulate development of specific antibodies in rabbits. Immunogen Injected Antibody Production in Serum Lkt50*—saline extract + Lkt50*—column extract + Mock injection - *Lkt50 is a smaller derivative of Lkt that lacks all hydrophobic regions. indicates at least 50 percent neutralization of toxicity of Lkt; indicates no neutralization activity. Source: Modified from Lee et al. (2001). Infect. and Immunity 69:5786–5793. What general conclusion can you draw from the data?
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Textbook Question
One of the major causes of sickness, death, and economic loss in the cattle industry is Mannheimia haemolytica, which causes bovine pasteurellosis, or shipping fever. Noninvasive delivery of a vaccine using transgenic plants expressing immunogens would reduce labor costs and trauma to livestock. An early step toward developing an edible vaccine is to determine whether an injected version of an antigen (usually a derivative of the pathogen) is capable of stimulating the development of antibodies in a test organism. The following table assesses the ability of a transgenic portion of a toxin (Lkt) of M. haemolytica to stimulate development of specific antibodies in rabbits. Immunogen Injected Antibody Production in Serum Lkt50*—saline extract + Lkt50*—column extract + Mock injection - *Lkt50 is a smaller derivative of Lkt that lacks all hydrophobic regions. indicates at least 50 percent neutralization of toxicity of Lkt; indicates no neutralization activity. Source: Modified from Lee et al. (2001). Infect. and Immunity 69:5786–5793. With regards to development of a usable edible vaccine, what work remains to be done?
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