Skip to main content
Pearson+ LogoPearson+ Logo
Ch. 20 - Recombinant DNA Technology
Back
Previous problem
Next problem
All textbooksKlug 12th EditionCh. 20 - Recombinant DNA TechnologyProblem 4

Chapter 19, Problem 4

The human insulin gene contains a number of sequences that are removed in the processing of the mRNA transcript. In spite of the fact that bacterial cells cannot excise these sequences from mRNA transcripts, explain how a gene like this can be cloned into a bacterial cell and produce insulin.

Verified Solution
Video duration:
1m
This video solution was recommended by our tutors as helpful for the problem above.
429
views
Was this helpful?

Video transcript

Hello everyone and welcome to today's video. So which of the following is our vector that contains the F one origin of replication of the F one page and can be replicated in a cell like a plasma. Well, let's go over each of our answer choices so that we may solve this problem. First of all, we have this echo or one will remember that when we see something like this, this are, is going to be standing for restriction enzyme. This is not going to be a vector or something that can be replicated. So we're going to cancel it up. Then we have Y A C and this is actually going to be standing for just artificial chromosome and it can actually enter the cell and be used for DNA replication just like a plasma. However, it is not going to contain this F one origin of replication of the F one of each. So we're going to cancel this out. Then we have this fage mid. Well, this fage mid is a vector that is going to be containing the F one origin of replication of the F one page, which is just what the question is asking about because of this, it can enter the sale and be replicated just like a plasma because of this. We're going to be highlighting answer choice C as a final answer to our question. I really hope this helped you and I hope to see you on the next one.
Previous problemNext problem
Related Practice
Textbook Question

Write a short essay or sketch a diagram that provides an overview of how recombinant DNA techniques help geneticists study genes.

255
views
Textbook Question

Why are most recombinant human proteins produced in animal or plant hosts instead of bacterial host cells?

307
views
Textbook Question

What roles do restriction enzymes, vectors, and host cells play in recombinant DNA studies? What role does DNA ligase perform in a DNA cloning experiment? How does the action of DNA ligase differ from the function of restriction enzymes?

274
views
Textbook Question
One of the major causes of sickness, death, and economic loss in the cattle industry is Mannheimia haemolytica, which causes bovine pasteurellosis, or shipping fever. Noninvasive delivery of a vaccine using transgenic plants expressing immunogens would reduce labor costs and trauma to livestock. An early step toward developing an edible vaccine is to determine whether an injected version of an antigen (usually a derivative of the pathogen) is capable of stimulating the development of antibodies in a test organism. The following table assesses the ability of a transgenic portion of a toxin (Lkt) of M. haemolytica to stimulate development of specific antibodies in rabbits. Immunogen Injected Antibody Production in Serum Lkt50*—saline extract + Lkt50*—column extract + Mock injection - *Lkt50 is a smaller derivative of Lkt that lacks all hydrophobic regions. indicates at least 50 percent neutralization of toxicity of Lkt; indicates no neutralization activity. Source: Modified from Lee et al. (2001). Infect. and Immunity 69:5786–5793. What general conclusion can you draw from the data?
290
views
Textbook Question
One of the major causes of sickness, death, and economic loss in the cattle industry is Mannheimia haemolytica, which causes bovine pasteurellosis, or shipping fever. Noninvasive delivery of a vaccine using transgenic plants expressing immunogens would reduce labor costs and trauma to livestock. An early step toward developing an edible vaccine is to determine whether an injected version of an antigen (usually a derivative of the pathogen) is capable of stimulating the development of antibodies in a test organism. The following table assesses the ability of a transgenic portion of a toxin (Lkt) of M. haemolytica to stimulate development of specific antibodies in rabbits. Immunogen Injected Antibody Production in Serum Lkt50*—saline extract + Lkt50*—column extract + Mock injection - *Lkt50 is a smaller derivative of Lkt that lacks all hydrophobic regions. indicates at least 50 percent neutralization of toxicity of Lkt; indicates no neutralization activity. Source: Modified from Lee et al. (2001). Infect. and Immunity 69:5786–5793. With regards to development of a usable edible vaccine, what work remains to be done?
265
views
Textbook Question

Although many cloning applications involve introducing recombinant DNA into bacterial host cells, many other cell types are also used as hosts for recombinant DNA. Why?

276
views