Skip to main content
Pearson+ LogoPearson+ Logo
Ch. 20 - Recombinant DNA Technology
Back
Previous problem
Next problem
All textbooksKlug 12th EditionCh. 20 - Recombinant DNA TechnologyProblem 5

Chapter 19, Problem 5

Although many cloning applications involve introducing recombinant DNA into bacterial host cells, many other cell types are also used as hosts for recombinant DNA. Why?

Verified Solution
Video duration:
2m
This video solution was recommended by our tutors as helpful for the problem above.
276
views
Was this helpful?

Video transcript

Hello everyone and welcome to today's video. So direct gene transfer is a method of introducing recombinant D N A into host cells, which of the following methods is not an example of direction transfer. And remember that interactions transfer, we only have the medium and the cell. There is nothing in between here that use directly transferring the D N A into the cell. So let's go over each of the answer. Choices. We have micro injection or micro injection uses a micro needle to transfer DNA directly into the nucleus by puncturing the plasma membrane. This is an example of direct genes transfer. So we're going to cancel it out, then we have been gone. And this is very interesting because it is going to be shooting these gold particles coated with DNA. And these are going to enter the cell being also a direct method of gene transfer. So we're going to cancel this. Remember that we're looking for the example that is not a a method of directions transfer. So keep that in mind, then we have electric operations. So we have cells in a medium, there's very high volts of electricity in the medium. So this is going to affect the plasma membrane allowing DNA to enter directly into the cell. We're also going to cancel it out because this is also a form of direct transfer and this is all leaving us with answer choice C or virus. So here we have, for example, a bacteria of each, this virus is going to be used to transfer the DNA into the cell. However, we're putting it inside the virus. So then then the virus can put it inside of the cell. So here we have a vector, we have a method that is not directly transferring the D N A from the medium into the cell. We have an intermediate because of this virus or the bacteria phages, which is answer choice C is going to be there. Correct answer to our question. I really hope this helped you and I hope to see you on the next one.
Previous problemNext problem
Related Practice
Textbook Question

The human insulin gene contains a number of sequences that are removed in the processing of the mRNA transcript. In spite of the fact that bacterial cells cannot excise these sequences from mRNA transcripts, explain how a gene like this can be cloned into a bacterial cell and produce insulin.

429
views
Textbook Question
One of the major causes of sickness, death, and economic loss in the cattle industry is Mannheimia haemolytica, which causes bovine pasteurellosis, or shipping fever. Noninvasive delivery of a vaccine using transgenic plants expressing immunogens would reduce labor costs and trauma to livestock. An early step toward developing an edible vaccine is to determine whether an injected version of an antigen (usually a derivative of the pathogen) is capable of stimulating the development of antibodies in a test organism. The following table assesses the ability of a transgenic portion of a toxin (Lkt) of M. haemolytica to stimulate development of specific antibodies in rabbits. Immunogen Injected Antibody Production in Serum Lkt50*—saline extract + Lkt50*—column extract + Mock injection - *Lkt50 is a smaller derivative of Lkt that lacks all hydrophobic regions. indicates at least 50 percent neutralization of toxicity of Lkt; indicates no neutralization activity. Source: Modified from Lee et al. (2001). Infect. and Immunity 69:5786–5793. What general conclusion can you draw from the data?
290
views
Textbook Question
One of the major causes of sickness, death, and economic loss in the cattle industry is Mannheimia haemolytica, which causes bovine pasteurellosis, or shipping fever. Noninvasive delivery of a vaccine using transgenic plants expressing immunogens would reduce labor costs and trauma to livestock. An early step toward developing an edible vaccine is to determine whether an injected version of an antigen (usually a derivative of the pathogen) is capable of stimulating the development of antibodies in a test organism. The following table assesses the ability of a transgenic portion of a toxin (Lkt) of M. haemolytica to stimulate development of specific antibodies in rabbits. Immunogen Injected Antibody Production in Serum Lkt50*—saline extract + Lkt50*—column extract + Mock injection - *Lkt50 is a smaller derivative of Lkt that lacks all hydrophobic regions. indicates at least 50 percent neutralization of toxicity of Lkt; indicates no neutralization activity. Source: Modified from Lee et al. (2001). Infect. and Immunity 69:5786–5793. With regards to development of a usable edible vaccine, what work remains to be done?
265
views
Textbook Question

Using DNA sequencing on a cloned DNA segment, you recover the nucleotide sequence shown below. Does this segment contain a palindromic recognition sequence for a restriction enzyme? If so, what is the double-stranded sequence of the palindrome, and what enzyme would cut at this sequence? (Consult Figure 20.1 for a list of restriction sites.)

CAGTATGGATCCCAT

293
views
Textbook Question

As genetic testing becomes widespread, medical records will contain the results of such testing. Who should have access to this information? Should employers, potential employers, or insurance companies be allowed to have this information? Would you favor or oppose having the government establish and maintain a central database containing the results of individuals' genome scans?

209
views
Textbook Question

Restriction sites are palindromic; that is, they read the same in the 5' to 3' direction on each strand of DNA. What is the advantage of having restriction sites organized this way?

409
views