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Ch. 20 - Recombinant DNA Technology

Chapter 19, Problem 7

Restriction sites are palindromic; that is, they read the same in the 5' to 3' direction on each strand of DNA. What is the advantage of having restriction sites organized this way?

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Hello, everyone. Here we have a question telling us. Restriction enzymes are proteins that cleave phospho dier bonds of DNA molecules as specific sites called palindromic sequences. Which of the following statements about restriction enzymes is correct. A different restriction enzymes that recognize the same sequence are known as neo schism. This is correct. B A bacterium uses a restriction enzyme to defend against bacterial viruses called bacterial phages. When a phage infects the bacterium, it inserts its DNA into the bacterial cell for its replication and proliferation. The restriction enzyme prevents replication of the phage DNA by cutting it into many pieces. So B is correct. See, the digestion of restriction enzymes results in sticky ends. A sticky end is produced when the restriction enzyme cuts at one end of the sequence between two bases on the same strand and then it goes on to cut the opposite end of the complementary strand. And what this does is produce two ends of DNA that will have some nucleotide without any complementary bases, which is known as sticky ends. So C is correct. So our answer here is D all of these. Thank you for watching. Bye.
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Although many cloning applications involve introducing recombinant DNA into bacterial host cells, many other cell types are also used as hosts for recombinant DNA. Why?

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Textbook Question

Using DNA sequencing on a cloned DNA segment, you recover the nucleotide sequence shown below. Does this segment contain a palindromic recognition sequence for a restriction enzyme? If so, what is the double-stranded sequence of the palindrome, and what enzyme would cut at this sequence? (Consult Figure 20.1 for a list of restriction sites.)

CAGTATGGATCCCAT

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