List and describe the function of the ten subunits constituting DNA polymerase III. Distinguish between the holoenzyme and the core enzyme.

Bacterial DNA polymerase I and DNA polymerase III perform different functions during DNA replication.

If a strain of E. coli acquired a mutation that inactivated DNA polymerase III function, would the cell be able to replicate its DNA? Why or why not?

Bacterial DNA polymerase I and DNA polymerase III perform different functions during DNA replication.

Identify the principal functions of each molecule.

Bacterial DNA polymerase I and DNA polymerase III perform different functions during DNA replication.

If mutation inactivated DNA polymerase I in a strain of E. coli, would the cell be able to replicate its DNA? If so, what kind of abnormalities would you expect to find in the cell?

Diagram a replication fork in bacterial DNA and label the following structures or molecules.

a. DNA pol III

b. Helicase

c. RNA primer

d. Origin of replication

e. leading strand (label its polarity)

f. DNA pol I

g. Topoisomerase

h. SSB protein

i. Lagging strand (label its polarity)

j. Primase

k. Okazaki fragment

List the proteins that unwind DNA during in vivo DNA synthesis. How do they function?

Define and indicate the significance of

(a) Okazaki fragments,

(b) DNA ligase, and

(c) primer RNA during DNA replication.

Outline the current model for DNA synthesis.