Chapter 13, Problem 17
Define the process of transcription. Where does this process fit into the central dogma of molecular biology (DNA makes RNA makes protein)?
Video transcript
Many promoter regions contain CAAT boxes containing consensus sequences CAAT or CCAAT approximately 70 to 80 bases upstream from the transcription start site. How might one determine the influence of CAAT boxes on the transcription rate of a given gene?
In principle, RNAi may be used to fight viral infection. How might this work?
Research indicates that promoters may fall into one of two classes: focused or dispersed. How do these classes differ, and which genes tend to be associated with each?
Explain the features of the Initiator (Inr) elements, BREs, DPEs, and MTEs of focused promoters.
What observations suggested the existence of mRNA?
Many transcriptional activators are proteins with a DNA-binding domain (DBD) and an activation domain (AD). Explain how each domain contributes to transcriptional initiation. Would you expect repressors to also have each of these domains?