What observations suggested the existence of mRNA?

Define the process of transcription. Where does this process fit into the central dogma of molecular biology (DNA makes RNA makes protein)?
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Central Dogma of Molecular Biology
Role of RNA in Protein Synthesis
Refer to the genetic coding dictionary to respond to the following:
A base-substitution mutation that altered the sequence shown in part (a) eliminated the synthesis of all but one polypeptide. The altered sequence is shown here:
5'-AUGCAUACCUAUGUGACCCUUGGA-3'
Determine why.
Most proteins have more leucine than histidine residues, but more histidine than tryptophan residues. Correlate the number of codons for these three amino acids with this information.
Shown here is a hypothetical viral mRNA sequence:
5'-AUGCAUACCUAUGAGACCCUUGGA-3'
Assuming that it could arise from overlapping genes, how many different polypeptide sequences can be produced? What are the sequences?
Write a paragraph describing the abbreviated chemical reactions that summarize RNA polymerase-directed transcription.
Describe the structure of RNA polymerase in bacteria. What is the core enzyme? What is the role of the σ subunit?
