Suppose that 22% of the nucleotides of a DNA molecule are deoxyadenosine and during replication the relative amounts of available deoxynucleoside triphosphates are 22% dATP, 22% dCTP, 28% dGTP, and 28% dTTP. What deoxynucleoside triphosphate is limiting to the replication? Explain.

Question

Accepted Answer

Hello, everyone. Today, we have the following problem. A newly discovered bacterium was found to have 31% of its DNA nucleotides to be guanine. During their application, the available relative DNTP amounts were found to be the following percentages which DDD TNT P is limiting to the replication of DNA. So first, we have to determine the percentage of the nucleotides in the DNA. So we know that there's 31% of the nucleotides are guanine. So since sardine is complimentary to guanine, that means there will also be 31% of cytosine because the total must equal 100% to determine the A MT of Anan. In thy mean, we take 100% and we actually subtract by the total content of guanine and cytosine. So that would be 31% for guanine plus 31% for cytosine for a total content of Guanyin. Andy is to be 62%. As though we take 100% subtract 62% to arrive at 38%. And this is the total for adding and diming. So we simply take that 38% divide by two such that we have 19% of adenine and 19% of thymine. And now we can compare the percentages with those of the DNTP. So we take Guan Yin, for example, the DNA, it was 31%. But for the DNTP or for its complimentary structure, it was 19%. And that would be the DC TP as cytosine is complimentary to guanine. So because this percentage is less than required to pair, we say that this molecule is limiting. We look at cytosine for the DNA was also 31%. And if we look at it's complimentary, the DGTP, it was 31%. So we say this is exact for Adenine in the DNA was 19% in the, as complimentary base pairing. It was also 19% as D TTP. And then we have thymine, which in the DNA was 19% as well. But in its complementary form, it was 31% of DATP and this is an excess. So to answer the question, the DC TP is limiting to the replication of DNA overall. I hope this helped. And until next time.

Suppose that 22% of the nucleotides of a DNA molecule are deoxyadenosine and during replication the relative amounts of available deoxynucleoside triphosphates are 22% dATP, 22% dCTP, 28% dGTP, and 28% dTTP. What deoxynucleoside triphosphate is limiting to the replication? Explain.

Verified Solution

Key Concepts

Nucleotide Composition

Deoxynucleoside Triphosphates (dNTPs)

Limiting Reagent