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Ch. 15 - Gene Mutation, DNA Repair, and Transposition

Chapter 15, Problem 12

Why are X rays more potent mutagens than UV radiation?

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Welcome back. Our next problem, says UV light induces mutations in DNA by. And when we look over our answer choices, we know the choice D. Is all of the above. So let's look through our answer choices. Choice A says helix distortion. Well, helix distortion isn't the method by which UV light induces mutation. Ah helix distortion is usually caused by inter cal eating agents. These inter cal eating agents have a ring like structure and they get inserted sort of wedged between DNA bases in the double helix, causing helix distortion. So that's not our correct answer and therefore Choice D. Is also incorrect since uh that was all of the above. And obviously this we already know that choice A is not correct. Choice B. Is forming perimeter timers. And this will be our answer. That is the method by which UV light induces mutations. Perimeter timers are caused when a perimeter eating the absorbed photon breaks open A double bond that a permitting has with its complementary base and causes it instead. If it has a neighboring promoting on its same strand causes it to form a bond with its neighbor perimeter. Obviously that's going to cause big issues in that D. N. A. Helix. If you've got a bond happening with your next door neighbor on the same strand versus the proper complementary pairing between the two strands. So that choice B. Is how UV light induces mutations. And then we have choice C. Creating free radicals. Well this is the main means by which ionizing radiation such as gamma rays or x rays causes mutations. The energy from ionizing radiation can knock off electrons, creating free radicals, creating these ions that can rampage about and break Covalin bonds and that obviously can cause DNA damage. So that's but that's the way ionizing radiation primarily induces mutations, not UV light. So choice C is not our answer. So again, UV light induces mutations in DNA. By choice. Be forming perimeter and timers see you in the next video.
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In studies of the amino acid sequence of wild-type and mutant forms of tryptophan synthetase in E. coli, the following changes have been observed: Determine a set of triplet codes in which only a single-nucleotide change produces each amino acid change.
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Contrast and compare the mutagenic effects of deaminating agents, alkylating agents, and base analogs.
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Why are frameshift mutations likely to be more detrimental than point mutations, in which a single pyrimidine or purine has been substituted?

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DNA damage brought on by a variety of natural and artificial agents elicits a wide variety of cellular responses involving numerous signaling pathways. In addition to the activation of DNA repair mechanisms, there can be activation of pathways leading to apoptosis (programmed cell death) and cell-cycle arrest. Why would apoptosis and cell-cycle arrest often be part of a cellular response to DNA damage?
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Textbook Question

A glycine residue is in position 210 of the tryptophan synthetase enzyme of wild-type E. coli. If the codon specifying glycine is GGA, how many single-base substitutions will result in an amino acid substitution at position 210? What are they? How many will result if the wild-type codon is GGU?

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Contrast the various types of DNA repair mechanisms known to counteract the effects of UV radiation. What is the role of visible light in repairing UV-induced mutations?
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