Why doesn't polynucleotide phosphorylase (Ochoa's enzyme) synthesize RNA in vivo?

Video transcript

Hi everybody. Let's take a look at this practice problem together. The following are the functions of pollen nucleotide phosphor lays except recall that pollen nucleotide phosphor lays is abbreviated P in P A. S. E. It is an enzyme. Remember that things that end in A. S. E. Are often enzymes. Now it's an enzyme that's involved in M. RNA processing of bacteria, plants and animals. And it has been researched both in vitro. So studies that are done outside of a living organism and in vivo, which are studies that are done inside a whole living organism. So we need to identify which of the options is not a function of P. And P. A. S. We have a. It can synthesize RNA A. Now this is a function of P. And P. A. S. This has been shown in vitro. It can act like a prelim arrays at the three prime end of RNA. To add on nuclear site die phosphates. Then we have B. It can degrade RNA. A. This is also a function. It has been shown in vitro as well. And by breaking apart the inorganic phosphate bonds, it's able to degrade RNA from the three prime end to the five prime end when inorganic phosphate is present. And then we have C. It can act as an exa nuclear's. Now this is also a function of P. And P. A. S. And this has been shown in vivo. So in living organisms it's been shown to act as an exa nuclear's when inorganic phosphate concentrations are high. So therefore our answer is going to be d. All options are correct. Alright, everyone. I hope you found this helpful, and I'll see you soon for the next practice problem.