Ch. 13 - The Genetic Code and Transcription
Chapter 13, Problem 2
Write a short essay describing how cis-acting regulatory elements, activators, and chromatin modifiers are all coordinately involved in regulating transcription initiation.
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Textbook Question
In this chapter, we focused on how eukaryotic genes are regulated at the transcriptional level. Along the way, we found many opportunities to consider the methods and reasoning by which much of this information was acquired. From the explanations given in the chapter,
How do we know that the orientation of promoters relative to the transcription start site is important while enhancers are orientation independent?
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Textbook Question
In this chapter, we focused on how eukaryotic genes are regulated at the transcriptional level. Along the way, we found many opportunities to consider the methods and reasoning by which much of this information was acquired. From the explanations given in the chapter,
How do we know that eukaryotic transcription factors bind to DNA sequences at or near promoter regions?
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Textbook Question
In this chapter, we focused on how eukaryotic genes are regulated at the transcriptional level. Along the way, we found many opportunities to consider the methods and reasoning by which much of this information was acquired. From the explanations given in the chapter,
How do we know that there is an association between disease susceptibility in humans and regulatory DNA sequences?
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Textbook Question
A number of experiments have demonstrated that areas of the genome that are transcriptionally inactive are also resistant to DNase I digestion. However, transcriptionally active areas are DNase I sensitive. Describe how DNase I resistance or sensitivity might indicate transcriptional activity.
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Textbook Question
Why doesn't polynucleotide phosphorylase (Ochoa's enzyme) synthesize RNA in vivo?
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Textbook Question
Refer to Table 13.1. Can you hypothesize why a synthetic RNA composed of a mixture of poly U poly A would not stimulate incorporation of ¹⁴C-phenylalanine into protein?
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