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Ch. 11 - DNA Replication and Recombination

Chapter 11, Problem 28

Assume that the sequence of bases shown below is present on one nucleotide chain of a DNA duplex and that the chain has opened up at a replication fork. Synthesis of an RNA primer occurs on this template starting at the base that is underlined. If the RNA primer consists of eight nucleotides, what is its base sequence?

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Hello everyone and welcome to today's video. So the sentences of an RNA primary begins at the base highlighted in the template below, which is going to be this timing that we have here, identify the sequence of the RNA primer that is nucleotides along. Well. In order to do this, we have to first extract the sequence of DNA that the RNA primer is coming for from. Remember that this RNA primer is going to be complementary to this D. N. A. Strand. Now, how are we going to do that? We're going to first. Right since this D. N. A strand is running three prime to five prime, we're going to be writing the opposite for or RNA primer. Now let's simply match or find a complementary nitrogenous base that would match with the nitrogenous bases in the D. N. A. Strength. Now let's do that. And this is where we get. Well remember first of all that in our any we do not have timing, we have euros L. So that is why we're going to be putting that is complementary to these at an inn base and let's continue. And eventually this is the RNA primer that we're going to be getting. Now if we look at our answer choices, we're going to see that the correct answer choice for this question is going to be answer choice. I really hope this video helped you and I hope to see you on the next one
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At the end of the short arm of human chromosome 16 (16p), several genes associated with disease are present, including thalassemia and polycystic kidney disease. When that region of chromosome 16 was sequenced, gene-coding regions were found to be very close to the telomere-associated sequences. Could there be a possible link between the location of these genes and the presence of the telomere-associated sequences? What further information concerning the disease genes would be useful in your analysis?
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Textbook Question
DNA polymerases in all organisms add only 5' nucleotides to the 3' end of a growing DNA strand, never to the 5' end. One possible reason for this is the fact that most DNA polymerases have a proofreading function that would not be energetically possible if DNA synthesis occurred in the 3' to 5' direction. Sketch the reaction that DNA polymerase would have to catalyze if DNA synthesis occurred in the 3' to 5' direction.
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Textbook Question
DNA polymerases in all organisms add only 5' nucleotides to the 3' end of a growing DNA strand, never to the 5' end. One possible reason for this is the fact that most DNA polymerases have a proofreading function that would not be energetically possible if DNA synthesis occurred in the 3' to 5' direction. Consider the information in your sketch and speculate as to why proofreading would be problematic.
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Textbook Question
Assume that the sequence of bases shown below is present on one nucleotide chain of a DNA duplex and that the chain has opened up at a replication fork. Synthesis of an RNA primer occurs on this template starting at the base that is underlined. In the intact RNA primer, which nucleotide has a free 3'-OH terminus? 3'.......GGCTACCTGGATTCA....5'
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Textbook Question
Reiji and Tuneko Okazaki conducted a now classic experiment in 1968 in which they discovered a population of short fragments synthesized during DNA replication. They introduced a short pulse of ³H-thymidine into a culture of E. coli and extracted DNA from the cells at various intervals. In analyzing the DNA after centrifugation in denaturing gradients, they noticed that as the interval between the time of ³H-thymidine introduction and the time of centrifugation increased, the proportion of short strands decreased and more labeled DNA was found in larger strands. What would account for this observation?
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