You are provided with cultures with the following characteristics:
Culture 1: F^+, genotype A^+B^+C^+
Culture 2: F ̄, genotype A ̄B ̄C ̄
a. Indicate the possible genotypes of a recombinant cell resulting from the conjugation of cultures 1 and 2.
b. Indicate the possible genotypes of a recombinant cell resulting from conjugation of the two cultures after the F^+ has become an Hfr cell.

Question

You are provided with cultures with the following characteristics:

Culture 1: F^+, genotype A^+B^+C^+

Culture 2: F ̄, genotype A ̄B ̄C ̄

a. Indicate the possible genotypes of a recombinant cell resulting from the conjugation of cultures 1 and 2.

b. Indicate the possible genotypes of a recombinant cell resulting from conjugation of the two cultures after the F^+ has become an Hfr cell.

Accepted Answer

Hey, everyone. Let's take a look at this question together if we mix an F plus and an F minus in a culture and bacterial conjugation occurs, which of the following can happen. Is it answer choice. A half of the resulting bacterial population will become an F plus strain. Answer choice B, all of the resulting bacterial population will become an F minus strain. Answer choice C, all of the resulting bacterial population will become an F plus strain or answer choice D, the number of F plus and F minus will depend on the nut added to the culture. Let's work this problem out together to try to figure out which of the following answer choices best describes what will occur when we mix an F plus and an F minus in a culture and bacterial conjugation occurs. So, in order to solve this question, we have to recall what we have learned about what bacterial conjugation is to determine what happens when we mix that F plus and F minus in the culture. And bacterial conjugation occurs. And we can recall that when F plus bacterial cells, which are the donor and the F minus bacterial cells. Which are the recipient are mixed in a culture and bacterial conjugation occurs. The F factor which is the F plasmid is transferred from the donor, which is the F plus bacterial cell to the recipient, which is the F minus bacterial cell. And as a result of that transfer of the F factor from the donor to the recipient, all recipient cells become F plus cells. So as a result, all of the resulting bacterial population will become an F plus strain, which is answer choice C. The correct answer since the mixing of that F plus bacterial cell, which is the donor with the F minus bacterial cell, which is the recipient. And the process of bacterial conjugation occurs the F factor which is that F plasmid is transferred from the donor to the recipient, resulting in all of the resulting bacterial population becoming an F plus strain. So answer choice C is the only correct answer choice. I hope you found this video to be helpful. Thank you and goodbye.

Verified Solution

Key Concepts

Conjugation in Bacteria

F Factor and Hfr Cells

Recombinant Genotypes