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Ch. 8 - Microbial Genetics
Chapter 8, Problem 8.6a

Identify when (before transcription, after transcription but before translation, after translation) each of the following regulatory mechanisms functions.
a. ATP combines with an enzyme, altering its shape.
b. A short RNA is synthesized that is complementary to mRNA.
c. Methylation of DNA occurs.
d. An inducer combines with a repressor.

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1
Identify the regulatory mechanism in each scenario.
a. ATP combines with an enzyme, altering its shape: This is an example of allosteric regulation, which typically occurs after translation.
b. A short RNA is synthesized that is complementary to mRNA: This describes RNA interference, which occurs after transcription but before translation.
c. Methylation of DNA occurs: DNA methylation is a form of epigenetic regulation that occurs before transcription.
d. An inducer combines with a repressor: This is a form of transcriptional regulation that occurs before transcription.

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Key Concepts

Here are the essential concepts you must grasp in order to answer the question correctly.

Enzyme Regulation

Enzyme regulation involves mechanisms that modify enzyme activity, often through the binding of molecules like ATP. This can lead to conformational changes in the enzyme, affecting its ability to catalyze reactions. Such regulation can occur before transcription, influencing the availability of substrates or the activity of transcription factors.
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RNA Interference

RNA interference (RNAi) is a biological process where short RNA molecules, such as small interfering RNA (siRNA), bind to complementary mRNA sequences. This binding can lead to mRNA degradation or inhibition of translation, effectively regulating gene expression after transcription but before translation.
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1) RNA Processing

DNA Methylation

DNA methylation is a chemical modification of DNA that typically occurs at cytosine bases, influencing gene expression. This process can silence genes and is a key regulatory mechanism that occurs before transcription, affecting the accessibility of DNA to transcription factors and RNA polymerase.
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DNA Methylation
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