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Ch. 8 - Microbial Genetics
Chapter 8, Problem 8.6a

Plasmids differ from transposons in that plasmids
a. become inserted into chromosomes.
b. are self-replicated outside the chromosome.
c. move from chromosome to chromosome.
d. carry genes for antibiotic resistance.
e. none of the above

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1
Understand the definitions: Plasmids are small, circular DNA molecules that exist independently of chromosomal DNA in bacteria. Transposons, also known as 'jumping genes', are DNA sequences that can change their position within the genome.
Identify the key characteristics of plasmids: They are capable of self-replication and often carry genes that can be beneficial to the host organism, such as antibiotic resistance genes.
Consider the options: (a) Plasmids do not typically integrate into chromosomes, unlike transposons. (b) Plasmids replicate independently of the chromosomal DNA. (c) Transposons are known for moving from one location to another within the genome, not plasmids. (d) While plasmids can carry antibiotic resistance genes, this is not a distinguishing feature from transposons.
Evaluate the options: Option (b) highlights the self-replicating nature of plasmids outside the chromosome, which is a key difference from transposons.
Conclude by selecting the option that best describes how plasmids differ from transposons, focusing on their ability to replicate independently of the chromosomal DNA.

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Key Concepts

Here are the essential concepts you must grasp in order to answer the question correctly.

Plasmids

Plasmids are small, circular DNA molecules that exist independently of chromosomal DNA in bacteria. They can replicate autonomously and often carry genes that provide advantageous traits, such as antibiotic resistance. Unlike chromosomal DNA, plasmids can be transferred between bacteria through processes like conjugation, enhancing genetic diversity.
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Transposons

Transposons, or 'jumping genes,' are segments of DNA that can move from one location to another within a genome. They can insert themselves into chromosomal DNA or plasmids, potentially disrupting genes and altering genetic function. Transposons play a significant role in genetic variation and evolution by facilitating the transfer of genetic material.
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Transposons in Prokaryotes

Antibiotic Resistance

Antibiotic resistance refers to the ability of bacteria to survive and grow in the presence of antibiotics that would normally inhibit or kill them. This resistance can be conferred by genes carried on plasmids, allowing bacteria to adapt quickly to antibiotic treatments. Understanding the mechanisms of resistance is crucial for developing effective therapies and managing bacterial infections.
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ABC Transporters Provide Multidrug Resistance
Related Practice
Textbook Question

Match the following terms to the definitions in questions 1 and 2.

a. conjugation

b. transcription

c. transduction

d. transformation

e. translation


Transfer of DNA from a donor to a recipient as naked DNA in solution.

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Textbook Question

Bacteria can acquire antibiotic resistance by all of the following except

a. mutation.

b. insertion of transposons.

c. conjugation.

d. snRNPs.

e. transformation.

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Textbook Question

Identify when (before transcription, after transcription but before translation, after translation) each of the following regulatory mechanisms functions.

a. ATP combines with an enzyme, altering its shape.

b. A short RNA is synthesized that is complementary to mRNA.

c. Methylation of DNA occurs.

d. An inducer combines with a repressor.

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Textbook Question

Use the following choices to answer questions 7 and 8:

a. catabolite repression

b. DNA polymerase

c. induction

d. repression

e. translation


Mechanism by which the presence of glucose inhibits the lac operon.

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Textbook Question

You are provided with cultures with the following characteristics:

Culture 1: F^+, genotype A^+B^+C^+

Culture 2: F ̄, genotype A ̄B ̄C ̄

a. Indicate the possible genotypes of a recombinant cell resulting from the conjugation of cultures 1 and 2.

b. Indicate the possible genotypes of a recombinant cell resulting from conjugation of the two cultures after the F^+ has become an Hfr cell.

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Textbook Question

Use the following choices to answer questions 7 and 8:

a. catabolite repression

b. DNA polymerase

c. induction

d. repression

e. translatio


The mechanism by which lactose controls the lac operon.

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