Phosphodiester Bond Formation - Video Tutorials & Practice Problems
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concept
Phosphodiester Bond Formation Concept 1
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Hey, everyone. So when we say phospho dier bond formation, we mean that the sugar of a nucleotide plus the phosphate group of another nucleotide equals an ester bond. Now, here we're going to say this is accomplished by the C three prime carbon oh group of one nucleotide. So this carbon in question and it's replacing an oxygen of the phosphate group and another nucleotide, right. So this oxygen here that I have highlighted, we use that one as the one we're replacing. But in actuality, it could be any of these oxygens, this one, this one or this one of the phosphate group. So here we'll just say this one. Now, what's going to happen is we're gonna draw this first nuclear tide to the left. And here is its PTOs ring. Just for simplicity, you write the nitrogenous group as just N in a box, we still have our C five prime carbon still connected to its phosphate group. And then this oxygen in oh it's going to replace this oxygen in purple that I circled and it's gonna be connected to the rest of this phosphate group. Here, this phosphate group is still connected to the rest of its nucleotide. So we're gonna have here, the ch 25 prime carbon of this nucleotide still connected to its until sugar with its oh on the three prime carbon and its nitrogenous base here. So here we're talking about our phospho disaster bonds. So we're really talking about the connections that we have here in reference to our fossil disaster bond formation. So we basically just linked up two nucleotides to the use of these phospho disaster bonds. All right. So that's the gist of what happens here in terms of when two nucleotides are reacting and we have to connect them together. It's in relation to the C three prime carbon oh of one nucleotide interacting with the phosphate group of another nucleotide.
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example
Phosphodiester Bond Formation Example 1
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In this example question, it says draw a fossil disaster bond formed from the following two nucleotides. Now remember this is accomplished by the oh on the C three prime carbon interact with one of the oxygens on the phosphate group of the other nucleotide. So here we're gonna draw this first nucleotide to the left. So we're gonna draw the penthos ring. We're gonna draw, it still has its five prime carbon connected to its phosphate group. Then we're gonna say that the oh that oxygen is going to link up with the phosphate group of the other nucleotide. So this phosphate group here because the connection it's connected still to its other oxygens. One of them being connected to its five prime carbon here, which is connected to its penthos ring. And here we have two ohs. Here you have an oh here. And all we have to do now is draw in the nitrogenous spaces. So here is our nitrogenous space for this first nucleotide and we're almost done, right? So then we have that with our alternating double bonds and our main group here. And then here goes the other one, same thing again, five ring with our alternating double bonds. And then our mean here at the top. So here we'd have our fossil disaster bond. We have our P this phosphate group that's linking both of our nucleotides together to give us our final structure here.
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Problem
Problem
Draw the following phosphodiester bond based on the given description: A phosphodiester bond that connects the C3’ OH group in the ribose of UMP and the 5’ carbon in the ribose of CMP.
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