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Ch. 5 - Chromosome Mapping in Eukaryotes
Chapter 5, Problem 26

In a certain plant, fruit is either red or yellow, and fruit shape is either oval or long. Red and oval are the dominant traits. Two plants, both heterozygous for these traits, were testcrossed, with the following results. Determine the location of the genes relative to one another and the genotypes of the two parental plants.

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Identify the traits and their dominance: Red (R) and Oval (O) are dominant, while Yellow (r) and Long (o) are recessive.
Determine the genotype of the heterozygous plants: Since both plants are heterozygous for both traits, their genotype is RrOo.
Perform a testcross: Cross the heterozygous plants (RrOo) with homozygous recessive plants (rroo) to observe the offspring phenotypes.
Analyze the offspring phenotypes: The phenotypic ratios will help determine if the genes are linked or assort independently.
If the phenotypic ratio deviates from the expected 1:1:1:1 ratio, the genes are likely linked. Calculate recombination frequency to determine the distance between the genes.

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Key Concepts

Here are the essential concepts you must grasp in order to answer the question correctly.

Dominance and Recessiveness

In genetics, dominance refers to the relationship between alleles, where the dominant allele masks the effect of the recessive allele in a heterozygous individual. In this scenario, red fruit color and oval shape are dominant traits, meaning that if a plant has at least one allele for red or oval, it will express those traits. Understanding this concept is crucial for predicting the phenotypes of offspring based on parental genotypes.
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Variations on Dominance

Heterozygosity and Testcrossing

Heterozygosity occurs when an individual has two different alleles for a particular gene, such as Rr for fruit color. A testcross involves breeding an individual of unknown genotype with a homozygous recessive individual to determine the unknown genotype based on the offspring's phenotypes. This method is essential for analyzing the genetic makeup of the plants in the question and understanding the inheritance patterns of the traits.
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Gene Linkage

Gene linkage refers to the tendency of genes located close to each other on the same chromosome to be inherited together during meiosis. If the traits for fruit color and shape are linked, the offspring's phenotypes will reflect this linkage, affecting the expected ratios. Analyzing the results of the testcross can reveal whether the genes for color and shape are linked or assort independently, which is vital for determining their relative locations on the chromosome.
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Related Practice
Textbook Question
Based on our discussion of the potential inaccuracy of mapping (see Figure 5.12), would you revise your answer to Problem 22? If so, how?
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Textbook Question
How do we know that in humans the X chromosomes play no role in human sex determination, while the Y chromosome causes maleness and its absence causes femaleness? Why are many expected crossover phenotypes missing? Can any of these loci be mapped from the data given here? If so, determine map distances.
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Textbook Question
Traditional gene mapping has been applied successfully to a variety of organisms including yeast, fungi, maize, and Drosophila. However, human gene mapping has only recently shared a similar spotlight. What factors have delayed the application of traditional gene-mapping techniques in humans?
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Textbook Question
Two plants in a cross were each heterozygous for two gene pairs (Ab/aB) whose loci are linked and 25 mu apart. Assuming that crossing over occurs during the formation of both male and female gametes and that the A and B alleles are dominant, determine the phenotypic ratio of their offspring.
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Textbook Question
A number of human–mouse somatic cell hybrid clones were examined for the expression of specific human genes and the presence of human chromosomes. The results are summarized in the following table. Assign each gene to the chromosome on which it is located.
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Textbook Question
A female of genotype a b c + + + produces 100 meiotic tetrads. Of these, 68 show no crossover events. Of the remaining 32, 20 show a crossover between a and b, 10 show a crossover between b and c, and 2 show a double crossover between a and b and between b and c. Of the 400 gametes produced, how many of each of the 8 different genotypes will be produced? Assuming the order a–b–c and the allele arrangement previously shown, what is the map distance between these loci?

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