A number of mouse models for human cystic fibrosis (CF) exist. Each of these mouse strains is transgenic and bears a different specific CFTR gene mutation. The mutations are the same as those seen in several varieties of human CF. These transgenic CF mice are being used to study the range of different phenotypes that characterize CF in humans. They are also used as models to test potential CF drugs. Unfortunately, most transgenic mouse CF strains do not show one of the most characteristic symptoms of human CF, that of lung congestion. Can you think of a reason why mouse CF strains do not display this symptom of human CF?

Video transcript

Hey, everyone. Let's take a look at this question together. The blank is a method that is used to create transgenic animals. This method involves the collection of fertilized eggs at the zygote stage. So let's take a look at the following answer choices to figure out which one best describes a method that is used to create transgenic animals involving the collection of fertilized eggs at the zygote stage. So the method that we're looking for that is used to create these transgenic animals, takes the male row nucleus of the fertilized news sites and it is injected with a trans gene containing solution and this solution is injected directly into the pro nucleus of these fertilized oocytes. And the name of this method that we use to create those transgenic animals is answer choice. A pro nuclear injection because in pro nuclear injection, a trans gene containing solution is injected directly into the male pro nucleus of these fertilized oocytes and then the eggs are then transferred to the over duct of the recipient mice. So answer choice. A pro nuclear injection is the correct answer. I hope you found this video to be helpful. Thank you. And Goodbye.