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Ch. 22 - Applications of Genetic Engineering and Biotechnology

Chapter 21, Problem 26

What techniques can scientists use to determine if a particular transgene has been integrated into the genome of an organism?

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Hi everyone welcome back. Let's look at our next problem blank Are organisms born from pseudo pregnant mice that have both normal and knockout stem cells. So let's kind of focus in on this that they have both normal and knockout stem cells. This means that the genome in the cells of this organism is not the same in every cell. So that's key there in distinguishing the term we want. So we have an organism in which different cells have a different genome from each other. So the term we want here is choice B. Chimeras coming from the creatures of legend that were made up of different you know the head of a bird and the tail of a lion. So in this case that reminds us that this animal is made up of cells with different genomes. So let's look at our other answer choices. To understand the distinction obviously Choice D. Is not correct. That's none of the options is correct. Since we have on the answer of chimera, choice A. Is transgenic organisms. And this might be a little confusing in terms of we might think this is our answer. Transgenic organisms are ones in which you have a gene or a specific piece of D. N. A. From one organism that's incorporated into the genome of another. However in transgenic organisms all cells have the same DNA. So that D. N. A. Does have DNA from another species incorporated but it's the same throughout the whole entire organism. Every cell the same genome as opposed to our crime eras are made up of cells with different genomes. So that's why is not correct mm choice E. Hybrids. Again, a hybrid being the result of the mating of two different species. So they have DNA from two different species. But again all cells have the same DNA. So they've inherited DNA from two different species in terms of their parental DNA, but the same throughout the whole organism. So that's why that's not a correct choice. So again, organisms born from pseudo pregnant mice that have both normal and knockout stem cells are Choice B cameras. See you in the next video.
Related Practice
Textbook Question

One complication of making a transgenic animal is that the transgene may integrate at random into the coding region, or the regulatory region, of an endogenous gene. What might be the consequences of such random integrations? How might this complicate genetic analysis of the transgene?

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Textbook Question

When disrupting a mouse gene by knockout, why is it desirable to breed mice until offspring homozygous (−/−) for the knockout target gene are obtained?

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Textbook Question

A number of mouse models for human cystic fibrosis (CF) exist. Each of these mouse strains is transgenic and bears a different specific CFTR gene mutation. The mutations are the same as those seen in several varieties of human CF. These transgenic CF mice are being used to study the range of different phenotypes that characterize CF in humans. They are also used as models to test potential CF drugs. Unfortunately, most transgenic mouse CF strains do not show one of the most characteristic symptoms of human CF, that of lung congestion. Can you think of a reason why mouse CF strains do not display this symptom of human CF?

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Textbook Question

Craig Venter and others have constructed synthetic copies of viral genomes. For example, the genome for poliovirus and the 1918 influenza strain responsible for the pandemic flu have been assembled this way. The United States currently has a moratorium on federal funding for 'gain-of-function' experiments which increase the virulence or transmission potential of viruses. What concerns might ethicists have about synthetic biology studies involving potential pandemic pathogens?

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Textbook Question

Gene targeting and gene editing are both techniques for removing or modifying a particular gene, each of which can produce the same ultimate goal. What is the main technical difference in how DNA is modified that differs between these approaches?

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