Gene targeting and gene editing are both techniques for removing or modifying a particular gene, each of which can produce the same ultimate goal. What is the main technical difference in how DNA is modified that differs between these approaches?

Video transcript

Welcome back. Let's look at our next problem blank is a molecular DNA and RNA analysis technique that separates DNA fragments and hybridize is them using labeled probes. So we're looking for a technique that involves these two parts, a separation of the D. N. A. Or RNA fragments. And that would be done by gel electrophoresis which separates our D. N. A. Or RNA fragments by size. And then hybridize them using labeled probes. So you have a probe with your complimentary to your sequence of interest that's labeled and you um run it over your fragments that have been separated and you can use that to analyze where your desired segment of interest is. So this process with these two parts is choice E southern blotting. And we'll just go through the other answer choices to understand why they're not correct. Choice A CRISPR is a technique for gene editing. So you're not just looking for a sequence of interest. You're finding your sequence of interest and actually physically cutting your D. N. A. There and either changing or replacing that gene. So it's obviously a lot more involved. You're actually physically changing the DNA, not just analyzing it. So Choice A is not correct Choice B fluorescence in situ hybridization. Uh It does have the aspect of your hybridizing desired sequences with labeled probes. In this case fluorescent probes are key. Here's the words in C. Two. Um This does this without the scent. The blotting without the gel doing an insight where they are located in the cells. So that's why that's not our correct answer. And finally, choice the stereotyping. Um, this allows examination of all the chromosomes so you physically see all the chromosomes. You can analyze them to see if there's any issues with any of them. And this is done by taking a cell sample and then steaming the cell to show visibly all of the chromosomes in the cell. So that's why that's not our correct answer either. So again, blank is a molecular DNA and RNA analysis technique that separates DNA fragments and hybridize is them using labeled probes and that's choice C southern blotting. See you in the next video.