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Ch. 21 - Genomic Analysis
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All textbooksKlug 12th EditionCh. 21 - Genomic AnalysisProblem 2

Chapter 20, Problem 2

Write a short essay that explains how recombinant DNA techniques were used to identify and study genes compared to how modern genomic techniques have revolutionized the cloning and analysis of genes.

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Hey, everyone. Let's take a look at this question together which of the following techniques is not utilized in recombinant DNA technology. So let's recall what we know about recombinant DNA technology to figure out which one of the following techniques is not utilized. So we know that recombinant DNA technology is the use of technology in which we are combining two or more fragments of DNA from different sources. And we are doing this through either DNA cloning as vector cloning or we use polymerase chain reaction or PC R. And so looking at the following techniques which one is not utilized. Well, we see we have DNA cloning, we have polymerase chain reaction and we have using cloning vector which we know that DNA cloning as vector cloning and PC R are all techniques utilized in recombinant DNA technology. So that means that the following technique that is not utilized is answer choice C shotgun sequencing, which is the correct answer because of the following techniques utilized in recomm in DNA technology. The only one here that is not utilized is shotgun sequencing which is answer choice C the correct answer. I hope you found this video to be helpful. Thank you and goodbye.
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Textbook Question

In this chapter, we focused on the analysis of genomes, transcriptomes, and proteomes and considered important applications and findings from these endeavors. At the same time, we found many opportunities to consider the methods and reasoning by which much of this information was acquired. From the explanations given in the chapter, what answers would you propose to the following fundamental questions?

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In this chapter, we focused on a number of interesting applications of genetic engineering, genomics, and biotechnology. At the same time, we found many opportunities to consider the methods and reasoning by which much of this information was acquired. From the explanations given in the chapter, what answers would you propose to the following fundamental questions? From microarray analysis how do we know what genes are being expressed in a specific tissue?
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Textbook Question
In this chapter, we focused on a number of interesting applications of genetic engineering, genomics, and biotechnology. At the same time, we found many opportunities to consider the methods and reasoning by which much of this information was acquired. From the explanations given in the chapter, what answers would you propose to the following fundamental questions? How can we correlate the genome with RNA expression data in a tissue or a single cell?
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What is functional genomics? How does it differ from comparative genomics?

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Compare and contrast WGS to a map-based cloning approach.

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