In this chapter, we focused on a number of interesting applications of genetic engineering, genomics, and biotechnology. At the same time, we found many opportunities to consider the methods and reasoning by which much of this information was acquired. From the explanations given in the chapter, what answers would you propose to the following fundamental questions? How can we correlate the genome with RNA expression data in a tissue or a single cell?

Video transcript

Hey, everyone. Let's take a look at this question together. Single cell sequencing involves isolating blank from a single cell and then subjecting it to whole genome amplification by PCR to produce sufficient DNA to be sequenced. Let's recall what we know about single cell sequencing to figure out what is isolated from that single cell and then subjected to whole genome amplification by PCR in order to produce sufficient D N A to be sequenced. So in order for us to do single cell sequencing, we have to isolate something from a single cell and then subjected to whole genome amplification by PCR to get that sufficient D N A sequence. And so in this process of single cell sequencing, what we are isolating from the single cell is its genomic DNA or answer choice C. The correct answer because single cell sequencing involves the isolation of the genomic DNA from a single cell and then subjecting it to whole genome amplification by PCR to produce sufficient DNA to be sequenced. So answer choice C genomic DNA is the correct answer. I hope you found this video to be helpful. Thank you and goodbye.