In Section 21.10 we briefly discussed the Human Proteome Map (HPM). An interactive Web site for the HPM is available at http://www.humanproteomemap.org. Visit this site, and then answer the questions in parts (a) and (b) and complete part (c). Use the 'Query' tab and select the 'Gene family' dropdown menu to do a search on the distribution of proteins encoded by a pathway of interest to you. Search in fetal tissues, adult tissues, or both.

Video transcript

Welcome back. Let's look at our next question. What were the primary tools used in the human proteome app? To analyze human proteins. And when we look at our answer choices, we have choice A liquid chromatography, choice be mass spectrometry, Choice C, options A and B. Or choice D. None of the options is correct. Well to analyze human proteins that involved the need to look at a sample from a cell to separate the proteins in the cell from each other and then to analyze the identity of the cells in that sample of the proteins in the sample. Excuse me. So to do that, you do need two separate tools to separate the proteins. The project used liquid chromatography that involves that column of absorbent material. You run your sample through it. The proteins interact differently with the materials in the column, causing them to have different flow rates and that actually separates out um in a very sensitive way the different proteins in a single sample. So liquid chromatography is used for separation of the proteins from each other. Then you need a way to identify the identity or to analyze the identity of the proteins in the sample. So for that they use choice be mass spectrometry um which is away then. So you have the proteins separated, you can take each individual protein sample, run it through the mass spectrometry and that will help give a clue to the identity of the protein by its mass. So since both A and B are correct, our answer choice is going to be see here options A and B. And obviously Choice D. None of the options is correct. Is not our answer. So again, what were the primary tools used in the human proteome app to analyze human proteins? Choice C, options A and B. See you in the next video.