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Ch. 21 - Genomic Analysis

Chapter 20, Problem 20

In Section 21.10 we briefly discussed the Human Proteome Map (HPM). An interactive Web site for the HPM is available at http://www.humanproteomemap.org. Visit this site, and then answer the questions in parts (a) and (b) and complete part (c). How many fetal tissues were analyzed?

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Hi, everyone. Let's look at our next question. It says based on the information available on the website www dot human proteome AP dot org, how many adult human tissues were used in the human proteome AP H P M? So let's take a minute to go to this website and look up right on the front page. There's information about how they compiled this map. So you can pause the video and look there. So let's recall what is what is a protium. So a proteome is the entire complement of proteins that can be expressed in an organism. So once the human genome project was complete, the proteome map was sort of the next step looking for what are the actual proteins. And of course, there are many, many, many more proteins that are expressed and there are genes due to alternative splicing, allowing many more proteins to be produced than there are genes in the genome. So because genes are expressed in different ways in different tissues and especially during development, as cells differentiate, um you have to look at a lot of different tissues and different stages of development. So our question asked how many adult human tissues were used. And when you look it up on that page, on the website, we see that there were 17 adult tissues, nine primary Hematopoietic hematopoietic cells and seven fetal tissues that were used as part of this map. So our answer here will be choice. C 17 adult tissues used in creating this map. See you in the next video.
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What are DNA microarrays? How are they used?

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Textbook Question
Annotation of the human genome sequence reveals a discrepancy between the number of protein-coding genes and the number of predicted proteins actually expressed by the genome. Proteomic analysis indicates that human cells are capable of synthesizing more than 100,000 different proteins and perhaps three times this number. What is the discrepancy, and how can it be reconciled?
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Textbook Question
In Section 21.10 we briefly discussed the Human Proteome Map (HPM). An interactive Web site for the HPM is available at http://www.humanproteomemap.org. Visit this site, and then answer the questions in parts (a) and (b) and complete part (c). How many proteins were identified in this project?
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Textbook Question
In Section 21.10 we briefly discussed the Human Proteome Map (HPM). An interactive Web site for the HPM is available at http://www.humanproteomemap.org. Visit this site, and then answer the questions in parts (a) and (b) and complete part (c). Use the 'Query' tab and select the 'Gene family' dropdown menu to do a search on the distribution of proteins encoded by a pathway of interest to you. Search in fetal tissues, adult tissues, or both.
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Textbook Question
Researchers have compared candidate loci in humans and rats in search of loci in the human genome that are likely to contribute to the constellation of factors leading to hypertension [Stoll, M., et al. (2000). Genome Res. 10:473–482]. Through this research, they identified 26 chromosomal regions that they consider likely to contain hypertension genes. How can comparative genomics aid in the identification of genes responsible for such a complex human disease? The researchers state that comparisons of rat and human candidate loci to those in the mouse may help validate their studies. Why might this be so?
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Textbook Question
Homology can be defined as the presence of common structures because of shared ancestry. Homology can involve genes, proteins, or anatomical structures. As a result of 'descent with modification,' many homologous structures have adapted different purposes. List three anatomical structures in vertebrates that are homologous but have different functions.
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