Chapter 13, Problem 16
Research indicates that promoters may fall into one of two classes: focused or dispersed. How do these classes differ, and which genes tend to be associated with each?
Video transcript
miRNAs target endogenous mRNAs in a sequence-specific manner. Explain, conceptually, how one might identify potential mRNA targets for a given miRNA if you only know the sequence of the miRNA and the sequence of all mRNAs in a cell or tissue of interest.
Many promoter regions contain CAAT boxes containing consensus sequences CAAT or CCAAT approximately 70 to 80 bases upstream from the transcription start site. How might one determine the influence of CAAT boxes on the transcription rate of a given gene?
In principle, RNAi may be used to fight viral infection. How might this work?
Define the process of transcription. Where does this process fit into the central dogma of molecular biology (DNA makes RNA makes protein)?
Explain the features of the Initiator (Inr) elements, BREs, DPEs, and MTEs of focused promoters.
What observations suggested the existence of mRNA?