What purpose do the bla and lacZ genes serve in the plasmid vecto...

Question

What purpose do the bla and lacZ genes serve in the plasmid vector pUC18?

Accepted Answer

Welcome back, everyone. Let's look at our next question. Which gene in a piece you see a plasmid is responsible for the selection of bacterial colonies transformed with a puc 18 plasmid. Choice A can Amycin resistance gene B, ampicillin resistance gene C tetracycline resistance gene or D chloro Phenocal resistance gene. Well, as we can recall, it's common, more plasmas used in cloning to carry a gene or the resistance to an antibiotic. Then bacteria that take up the gene are resistant to the antibiotic, treat a plate with antibiotic and you'll only have bacterial colonies that have taken up the plasmid in the case of puc 18, that is choice B an ampicillin resistant gene. They have a gene for the enzyme beta la which does confer that resistance to ampicillin. So treat your plate with epic and bacteria without the plasmin die. And you just have the bacteria that have been transformed and have taken up that plasmid. Our other answer choices. Uh Choice A Canam Mycin C Tetracycline and D chloramphenicol are all antibiotics that can be used. Uh genes for resistance to them are used in other uh cloning applications, but not specifically with the puc 18 plasmid that carries the choice B ampicillin resistance stream. See you in the next video.

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What purpose do the bla and lacZ genes serve in the plasmid vector pUC18?