Number the following steps of protein synthesis in the order in which they occur, starting with 1 and ending with 9.

a._____The stop codon is reached, and the polypeptide is released.          

b. _____The small ribosomal subunit finds the start codon, and the large ribosomal subunit joins.          

c. _____The end of the gene is reached, and the pre-mRNA is released and then edited.          

d. _____The transcription factor binds the promoter.          

e. _____The protein is folded and modified to become functional.          

f. _____ RNA polymerase builds the mRNA transcript.          

g. _____mRNA and initiator tRNA bind the small ribosomal subunit.          

h. _____ New tRNAs are brought into the A site successively, and the peptide chain of the tRNA in the P site is joined to the amino acid of the tRNA in the A site.

i. ______mRNA exits the nucleus via a nuclear pore.

Video transcript

Hi everyone. Let's take a look at this practice problem together which of the following events is considered a post translational modification. The answer options are a, the removal of rons through splicing B. The synthesis of RN A molecules by the RN A polymerase C. The unwinding of the DNA double helix by the RN A polymerase and D the binding of appropriate amino acid to the MRN ASE codon. So recall that translation is the process where ribosomes produce polypeptides using MRN A as a template. So translation is polypeptide synthesis and post translational modifications would occur on those polypeptides after translation occurs. Posttranslational modifications are done to produce structurally functional proteins. Now, knowing this information, we should recognize that we can eliminate options. BC and D. Option B. The synthesis of RN A molecules by the RN A polymeric. This occurs during transcription. So this is transcription and this happens before translation can begin. Option C, the unwinding of DNA it occurs so that transcription can begin. So this also occurs before translation. Option D, the binding of an amino acid to codon. This occurs during translation. So all three answer options, BC and D occur before or during translation. The correct answer therefore, is splicing of the introns. Now recall that the introns are the non coding regions. And so they must be removed before a protein can be functional. So the correct answer is option a the removal of rons through splicing. All right, everyone. I hope you found this helpful and I'll see you soon for the next practice problem.