Which of the following is the fourth basic step to genetically modify a cell?
a. transformation
b. ligation
c. plasmid cleavage
d. restriction-enzyme digestion of gene
e. isolation of gene

Question

Which of the following is the fourth basic step to genetically modify a cell?

a. transformation

b. ligation

c. plasmid cleavage

d. restriction-enzyme digestion of gene

e. isolation of gene

Accepted Answer

Hey, everyone. Let's take a look at this question together. All of the following are the processes involved in the genetic modification of a cell, except which is it answer choice. A gene isolation. Answer choice B gene insertion into a vector answer choice C DNA cleavage or answer choice D DNA unwinding. Let's work this problem out together to try to figure out which of the following answer. Choices is a process that is not involved in the genetic modification of a cell. So in order to solve this question, we have to recall what we have learned about each of the following types of processes to determine which is a process that is not involved in the genetic modification of a cell. And when we are talking about that genetic modification of a cell, we are talking about a process that is involved indirectly manipulating and recombining DNA sequences. So looking at our answer choices, starting off with answer choice. A, we have gene isolation which we know that the process of gene isolation involves isolating the gene of interest from its natural source, which is a process that is involved in the genetic modification of a cell which makes answer choice. A, an incorrect answer. Next, we have answer choice B which says the gene in insertion into a vector, which we know that the process of inserting a gene into a vector is also a process involved in the genetic modification of a cell. Since the gene of interest is typically inserted into a vector, which serves as the carrier to deliver the gene into the target, which makes answer choice B incorrect as well. And then we have answer choice C which says DNA, which we know that the process of DNA cleavage uses enzymes to cut the DNA so that it can be manipulated and combined. So it is also a process that is involved in the genetic modification of a cell making answer choice C incorrect, which leaves us with only answer choice D as the remaining answer choice, which says DNA unwinding, which we know that the process of DNA unwinding involves the separation of the DNA double helix into two separate strands allowing for the synthesis of the new DNA or RN A. And while it is crucial for cellular processes, it is not directly part of the genetic modification process. So answer choice D is the correct answer. I hope you found this video to be helpful. Thank you and goodbye.

Which of the following is the fourth basic step to genetically modify a cell?
a. transformation
b. ligation
c. plasmid cleavage
d. restriction-enzyme digestion of gene
e. isolation of gene

Verified Solution

Key Concepts

Genetic Modification Steps

Restriction Enzymes

Transformation

Which of the following is the fourth basic step to genetically modify a cell?a. transformationb. ligationc. plasmid cleavaged. restriction-enzyme digestion of genee. isolation of gene

Verified Solution

Key Concepts

Genetic Modification Steps

Restriction Enzymes

Transformation

Which of the following is the fourth basic step to genetically modify a cell?
a. transformation
b. ligation
c. plasmid cleavage
d. restriction-enzyme digestion of gene
e. isolation of gene