Skip to main content
Ch. 8 - Microbial Genetics
Chapter 8, Problem 9.5a

If you put a gene in a virus, the next step in genetic modification would be
a. insertion of a plasmid.
b. transformation.
c. transduction.
d. PCR.
e. Southern blotting.

Verified step by step guidance
1
Identify the process of inserting a gene into a virus, which is often used in genetic engineering to introduce new genetic material into a host cell.
Understand that the next step involves transferring the genetic material from the virus to the host cell.
Consider the method of transferring genetic material using a virus, which is known as transduction.
Review the definitions of the options provided: insertion of a plasmid, transformation, transduction, PCR, and Southern blotting.
Determine which option aligns with the process of using a virus to introduce genetic material into a host cell, focusing on the concept of transduction.

Verified Solution

Video duration:
2m
This video solution was recommended by our tutors as helpful for the problem above.
Was this helpful?

Key Concepts

Here are the essential concepts you must grasp in order to answer the question correctly.

Genetic Modification

Genetic modification involves altering the genetic material of an organism to achieve desired traits. This can be done through various techniques, including the insertion of genes into vectors like viruses, which can then deliver these genes into host cells. Understanding the methods of genetic modification is crucial for determining the next steps after gene insertion.
Recommended video:

Transduction

Transduction is a process by which foreign DNA is introduced into a bacterial cell via a virus (bacteriophage). In the context of genetic modification, if a gene is inserted into a virus, transduction would be the method by which that gene is transferred into the target organism's cells. This concept is essential for understanding how viral vectors can be used in genetic engineering.
Recommended video:

Plasmids

Plasmids are small, circular DNA molecules found in bacteria that can replicate independently of chromosomal DNA. They are often used as vectors in genetic engineering to carry and introduce foreign genes into host cells. The mention of plasmid insertion in the question highlights the alternative methods of gene delivery, which are important to consider in the context of genetic modification.
Recommended video: