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Ch. 8 - Microbial Genetics
Chapter 8, Problem 9.10a

Match the following choices to the statements in questions 7 through 10.
a. antisense
b. clone
c. library
d. Southern blot
e. vector


DNA that hybridizes with mRNA.

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1
Identify the key terms in the statement: 'DNA that hybridizes with mRNA'.
Understand that 'hybridizes' refers to the process where two complementary strands of nucleic acids bind together.
Recall that mRNA is a single-stranded molecule that carries genetic information from DNA to the ribosome.
Consider the role of antisense DNA, which is complementary to mRNA and can bind to it, preventing translation.
Match the term 'antisense' with the statement, as it describes DNA that can hybridize with mRNA.

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Key Concepts

Here are the essential concepts you must grasp in order to answer the question correctly.

Antisense RNA

Antisense RNA refers to a strand of RNA that is complementary to a given mRNA strand. It can hybridize with the mRNA, effectively blocking its translation into protein. This concept is crucial in gene regulation and is often used in research and therapeutic applications to inhibit gene expression.
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1) RNA Processing

Hybridization

Hybridization is the process by which two complementary strands of nucleic acids, such as DNA and RNA, bind together to form a double-stranded structure. This principle is fundamental in molecular biology techniques, including the detection of specific RNA molecules using antisense probes, which can be used in various applications like diagnostics and research.
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Overview of Transcription

mRNA

Messenger RNA (mRNA) is a type of RNA that carries genetic information from DNA to the ribosome, where proteins are synthesized. Understanding mRNA is essential for grasping how genes are expressed and regulated, as well as the role of antisense RNA in modulating this process by binding to mRNA and preventing its translation.
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Monocistronic mRNA vs. Polycistronic mRNA