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Ch. 5 - Microbial Metabolism
Chapter 5, Problem 5.5a

There are three mechanisms for the phosphorylation of ADP to produce ATP. Write the name of the mechanism that describes each of the reactions in the following table.
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Identify the three main mechanisms for ATP production: substrate-level phosphorylation, oxidative phosphorylation, and photophosphorylation.
Substrate-level phosphorylation occurs directly in metabolic pathways such as glycolysis and the citric acid cycle, where a phosphate group is directly transferred to ADP from a phosphorylated intermediate.
Oxidative phosphorylation takes place in the mitochondria, where ATP is produced using energy derived from the electron transport chain and a proton gradient across the inner mitochondrial membrane.
Photophosphorylation occurs in chloroplasts during photosynthesis, where light energy is used to create a proton gradient that drives ATP synthesis.
Match each reaction in the table to one of these mechanisms based on the context and location of the reaction, such as whether it occurs in the mitochondria, chloroplasts, or during glycolysis.

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Key Concepts

Here are the essential concepts you must grasp in order to answer the question correctly.

Substrate-Level Phosphorylation

Substrate-level phosphorylation is a direct method of generating ATP by transferring a phosphate group from a high-energy substrate to ADP. This process occurs in the cytoplasm during glycolysis and in the mitochondria during the Krebs cycle, where specific enzymes facilitate the transfer of the phosphate group, resulting in ATP production without the need for an electron transport chain.
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Substrate-Level Phosphorylation

Oxidative Phosphorylation

Oxidative phosphorylation is the process by which ATP is produced using energy derived from the electron transport chain, which is located in the inner mitochondrial membrane. Electrons from NADH and FADH2 are transferred through a series of protein complexes, creating a proton gradient that drives ATP synthesis via ATP synthase. This mechanism is crucial for aerobic respiration and generates the majority of ATP in cells.
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Oxidative Phosphorylation

Photophosphorylation

Photophosphorylation is the process of ATP formation that occurs in photosynthetic organisms, utilizing light energy. In this mechanism, light excites electrons in chlorophyll, leading to the creation of a proton gradient across the thylakoid membrane in chloroplasts. This gradient powers ATP synthase to convert ADP and inorganic phosphate into ATP, playing a vital role in the light-dependent reactions of photosynthesis.
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Related Practice
Textbook Question

Which of the following processes does not generate ATP?

a. photophosphorylation

b. the Calvin-Benson cycle

c. oxidative phosphorylation

d. substrate-level phosphorylation

e. All of the above generate ATP

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Textbook Question

Which of the following compounds has the greatest amount of energy for a cell?

a. CO₂

b. ATP

c. glucose

d. O₂

e. lactic acid

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Textbook Question

Define oxidation-reduction, and differentiate the following terms:

a. aerobic and anaerobic respiration

b. respiration and fermentation

c. cyclic and noncyclic photophosphorylation

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Textbook Question

Which of the following is the best definition of the Krebs cycle?

a. the oxidation of pyruvic acid

b. the way cells produce CO₂

c. a series of chemical reactions in which NADH is produced from the oxidation of pyruvic acid

d. a method of producing ATP by phosphorylating ADP

e. a series of chemical reactions in which ATP is produced from the oxidation of pyruvic acid

130
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Textbook Question

All of the energy-producing biochemical reactions that occur in cells, such as photophosphorylation and glycolysis, are ________ reactions.

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Textbook Question

Which of the following is the best definition of cellular respiration?

a. a sequence of redox reactions with O₂ as the final electron acceptor

b. a sequence of redox reactions with the final electron acceptor from the environment

c. a method of generating ATP

d. the complete oxidation of glucose to CO₂ and H₂O

e. a series of reactions in which pyruvic acid is oxidized to CO₂ and H₂O

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