Ch. 6 - Genetic Analysis and Mapping in Bacteria and Bacteriophages
All textbooksKlug 12th EditionCh. 6 - Genetic Analysis and Mapping in Bacteria and BacteriophagesProblem 3
Chapter 6, Problem 3
Price et al. [(1999). J. Bacteriol. 181:2358–2362] conducted a genetic study of the toxin transport protein (PA) of Bacillus anthracis, the bacterium that causes anthrax in humans. Within the 2294-nucleotide gene in 26 strains they identified five point mutations—two missense and three synonyms—among different isolates. Necropsy samples from an anthrax outbreak in 1979 revealed a novel missense mutation and five unique nucleotide changes among ten victims. The authors concluded that these data indicate little or no horizontal transfer between different B. anthracis strains.
What is meant by 'horizontal transfer'?
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Textbook Question
In this chapter, we have focused on genetic systems present in bacteria and on the viruses that use bacteria as hosts (bacteriophages). In particular, we discussed mechanisms by which bacteria and their phages undergo genetic recombination, which allows geneticists to map bacterial and bacteriophage chromosomes. In the process, we found many opportunities to consider how this information was acquired. From the explanations given in the chapter, what answers would you propose to the following questions?
How do we know that genes exist in bacteria and bacteriophages?
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Textbook Question
In this chapter, we have focused on genetic systems present in bacteria and on the viruses that use bacteria as hosts (bacteriophages). In particular, we discussed mechanisms by which bacteria and their phages undergo genetic recombination, which allows geneticists to map bacterial and bacteriophage chromosomes. In the process, we found many opportunities to consider how this information was acquired. From the explanations given in the chapter, what answers would you propose to the following questions?
How do we know that bacteria undergo genetic recombination, allowing the transfer of genes from one organism to another?
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Textbook Question
Write a short summary that contrasts how recombination occurs in bacteria and bacteriophages.
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Textbook Question
With respect to F⁺ and F⁻ bacterial matings, answer the following questions:
How was it established that physical contact between cells was necessary?
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Textbook Question
With respect to F⁺ and F⁻ bacterial matings, answer the following questions:
How was it established that chromosome transfer was unidirectional?
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Textbook Question
With respect to F⁺ and F⁻ bacterial matings, answer the following questions:
What is the genetic basis for a bacterium's being F⁺.
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