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Ch. 6 - Genetic Analysis and Mapping in Bacteria and Bacteriophages

Chapter 6, Problem 2

Write a short summary that contrasts how recombination occurs in bacteria and bacteriophages.

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Hello everyone welcome back. Here's our next question. It says the uptake of D. N. A. From the external environment by a bacterial cell is called blink. We've got terms here transformation. Conjugation. Transact transaction. And so ginny. And this question is just a little bit tricky because each of these processes involves um transfer of D. N. A. From somewhere to a bacterial cell. So we need to keep straight that we're looking for the uptake of D. N. A. From the external environment. And that is actually gonna be our first answer choice. A transformation because transformation refers this uptake of what we might call naked D. N. A. So it's not in another organism it's just loose D. N. A. In the external environment and it can get taken in by the bacterial cell and incorporated into the bacteria's genome. So that is our phrase we're looking for. But let's look at our other answer choices to understand why they're not correct. Choice B. Is conjugation and conjugation. Um Again you do have the uptake of genetic information by a bacterium. But in conjugation you have DNA transfer between two different bacterial cells. Um you have the two cells contact each other fused together and DNA is exchange between them. So it's not just picking up DNA from external environment. You have been transferred from one bacterial cell to another. So that's why that's not our correct answer. Then we have choice C transaction. I'm just gonna add a little arrow here to my definition for trans reduction and trans deduction is that process of a bacterial fade or virus transferring DNA into a bacterium. So again bacteria is taken in foreign D. N. A. But in this case it's being transferred the bacteria from a bacteria fage. So not quite the term we're looking for. And finally we have choice D. List so ginny and again I'm going to draws a little arrow here. Your definition misogyny is one of the two cycles of viral reproduction and involves the integration of the viral genome into the host bacteria's genome. So we no viruses don't reproduce on their own. They reproduce by hijacking the mechanism of their host cell. And misogyny is that stage in the virus life cycle where its genome is incorporated into the bacteria's genome to be replicated along with the bacteria's own DNA. Um So taking up of bacteria taking up of DNA by the bacteria but not just from the external environment generally. So not the term we're looking for. So again the uptake of DNA from the external environment by a bacterial cell such as DNA by itself is called choice. A transformation in the next video
Related Practice
Textbook Question
In this chapter, we have focused on genetic systems present in bacteria and on the viruses that use bacteria as hosts (bacteriophages). In particular, we discussed mechanisms by which bacteria and their phages undergo genetic recombination, which allows geneticists to map bacterial and bacteriophage chromosomes. In the process, we found many opportunities to consider how this information was acquired. From the explanations given in the chapter, what answers would you propose to the following questions? How do we know that bacteriophages recombine genetic material through transduction and that cell-to-cell contact is not essential for transduction to occur?
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Textbook Question
In this chapter, we have focused on genetic systems present in bacteria and on the viruses that use bacteria as hosts (bacteriophages). In particular, we discussed mechanisms by which bacteria and their phages undergo genetic recombination, which allows geneticists to map bacterial and bacteriophage chromosomes. In the process, we found many opportunities to consider how this information was acquired. From the explanations given in the chapter, what answers would you propose to the following questions? How do we know that genes exist in bacteria and bacteriophages?
203
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Textbook Question
In this chapter, we have focused on genetic systems present in bacteria and on the viruses that use bacteria as hosts (bacteriophages). In particular, we discussed mechanisms by which bacteria and their phages undergo genetic recombination, which allows geneticists to map bacterial and bacteriophage chromosomes. In the process, we found many opportunities to consider how this information was acquired. From the explanations given in the chapter, what answers would you propose to the following questions? How do we know that bacteria undergo genetic recombination, allowing the transfer of genes from one organism to another?
223
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Textbook Question

Price et al. [(1999). J. Bacteriol. 181:2358–2362] conducted a genetic study of the toxin transport protein (PA) of Bacillus anthracis, the bacterium that causes anthrax in humans. Within the 2294-nucleotide gene in 26 strains they identified five point mutations—two missense and three synonyms—among different isolates. Necropsy samples from an anthrax outbreak in 1979 revealed a novel missense mutation and five unique nucleotide changes among ten victims. The authors concluded that these data indicate little or no horizontal transfer between different B. anthracis strains.

What is meant by 'horizontal transfer'?

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Textbook Question
With respect to F⁺ and F⁻ bacterial matings, answer the following questions: How was it established that physical contact between cells was necessary?
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Textbook Question
With respect to F⁺ and F⁻ bacterial matings, answer the following questions: How was it established that chromosome transfer was unidirectional?
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