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Ch. 6 - Genetic Analysis and Mapping in Bacteria and Bacteriophages
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All textbooksKlug 12th EditionCh. 6 - Genetic Analysis and Mapping in Bacteria and BacteriophagesProblem 3

Chapter 6, Problem 3

With respect to F⁺ and F⁻ bacterial matings, answer the following questions: What is the genetic basis for a bacterium's being F⁺.

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Hello everyone here we have a question telling us blank. Is the sex factor in bacteria? We have a chromosome replicant chromosome. Or replicant is defined as the entire region of D. N. A. That is independently replicated from a single origin of replication. So A. Is incorrect. B. F pilots There are two types of bacterial cells F. Positive and negative. The F. Positive has a circular plasmid that contains the sex factor gene. The growth of F. Pillai which is involved in the conjugation process in the lateral transfer of genes is facilitated by the sex factor F. Positive. So Option B. Is our answer. But let's look at C. RNA. RNA is the genetic material for many viruses bacteria do have RNA but it does not act as the genetic material in them and it is not the sex factor in bacteria. So C. Is incorrect. And since B is our correct answer, D. Is incorrect. So our answer is B. F. Pilots thank you for watching. Bye.
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Textbook Question

Price et al. [(1999). J. Bacteriol. 181:2358–2362] conducted a genetic study of the toxin transport protein (PA) of Bacillus anthracis, the bacterium that causes anthrax in humans. Within the 2294-nucleotide gene in 26 strains they identified five point mutations—two missense and three synonyms—among different isolates. Necropsy samples from an anthrax outbreak in 1979 revealed a novel missense mutation and five unique nucleotide changes among ten victims. The authors concluded that these data indicate little or no horizontal transfer between different B. anthracis strains.

What is meant by 'horizontal transfer'?

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Textbook Question
With respect to F⁺ and F⁻ bacterial matings, answer the following questions: How was it established that physical contact between cells was necessary?
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Textbook Question
With respect to F⁺ and F⁻ bacterial matings, answer the following questions: How was it established that chromosome transfer was unidirectional?
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Textbook Question
List all major differences between (a) the F⁺ x F⁻ and the Hfr x F⁻ bacterial crosses; and (b) the F⁺, F⁻, Hfr, and F' bacteria.
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Textbook Question
Describe the basis for chromosome mapping in the Hfr x F⁻ crosses.
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Textbook Question
In general, when recombination experiments are conducted with bacteria, participating bacteria are mixed in complete medium, then transferred to a minimal growth medium. Why isn't the protocol reversed: minimal medium first, complete medium second?
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