Traditional Sanger sequencing has largely been replaced in recent years by next-generation and third-generation sequencing approaches. Describe advantages of these sequencing methods over first-generation Sanger sequencing.

Video transcript

Hello everyone here we have a question telling. A singer technique is also called Died. The oxy chain termination sequencing since it utilizes modified deoxyribonucleic tides called died the oxy nucleotides wire died. The oxy nucleotides consider chain termination. Nucleotide di di oxy nuclear ties, also known as D. D. N. T. P. S. Are considered chain termination nucleotides because they lack the three prime oxygen, which is critical to form a phosphate ester bond with another nucleotide. So our answer here is B. They lack the three prime oxygen. If D. D. N. T. P. S. Are included in the D. N. A synthesis reaction, the D D N. T. P. Will be added and DNA synthesis will no longer be able to continue because new new flu ties can no longer be added. So our answer is again be they lack the three prime oxygen. Thank you for watching. Bye.