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Ch. 20 - Recombinant DNA Technology
All textbooksKlug 12th EditionCh. 20 - Recombinant DNA TechnologyProblem 22
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Chapter 19, Problem 22

How is fluorescent in situ hybridization (FISH) used to produce a spectral karyotype?

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Hello, everyone here. We have a question asking how does the spectral carry A type differ from the traditional stereotype A. It provides a larger photograph. This is incorrect, which makes deep incorrect B. It gives each chromosome a different color. This is correct. In spectral carry A type. Each chromosome is detected using probes that are labeled with eyes that will fluoresce at different wavelengths, giving each individual chromosome different colors. So our answer here is B. Thank you for watching. Bye.
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Textbook Question
Microsatellites are currently exploited as markers for paternity testing. A sample paternity test is shown in the following table in which ten microsatellite markers were used to test samples from a mother, her child, and an alleged father. The name of the microsatellite locus is given in the left-hand column, and the genotype of each individual is recorded as the number of repeats he or she carries at that locus. For example, at locus D9S302, the mother carries 30 repeats on one of her chromosomes and 31 on the other. In cases where an individual carries the same number of repeats on both chromosomes, only a single number is recorded. (Some of the numbers are followed by a decimal point, for example, 20.2, to indicate a partial repeat in addition to the complete repeats.) Assuming that these markers are inherited in a simple Mendelian fashion, can the alleged father be excluded as the source of the sperm that produced the child? Why or why not? Explain.
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As you will learn later in the text (Special Topics Chapter 1— CRISPR-Cas and Genome Editing), the CRISPR-Cas system has great potential but also raises many ethical issues about its potential applications because theoretically it can be used to edit any gene in the genome. What do you think are some of the concerns about the use of CRISPR-Cas on humans? Should CRISPR-Cas applications be limited for use on only certain human genes but not others? Explain your answers.

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