In the triplet binding technique, radioactivity remains on the filter when the amino acid corresponding to the codon is labeled. Explain the rationale for this technique.

Verified step by step guidance

1. The triplet binding technique is used to determine which amino acids correspond to specific codons in the genetic code.
2. In this technique, a synthetic RNA triplet (codon) is used to bind to ribosomes, forming a complex that can attract the corresponding aminoacyl-tRNA.
3. The aminoacyl-tRNA is labeled with a radioactive marker, allowing it to be detected when it binds to the ribosome-RNA complex.
4. When the correct aminoacyl-tRNA binds to the ribosome-RNA complex, the radioactivity is retained on the filter, indicating a successful match between the codon and the amino acid.
5. This method helps in deciphering the genetic code by identifying which amino acid is specified by each codon.

Key Concepts

Triplet Binding Technique

The triplet binding technique is a method used to study the interaction between mRNA codons and their corresponding aminoacyl-tRNA during protein synthesis. In this technique, a specific codon is presented, and the amino acid that matches this codon is labeled with a radioactive isotope. This allows researchers to track which amino acids are incorporated into the growing polypeptide chain, providing insights into the translation process.

Codon-Amino Acid Relationship

Codons are sequences of three nucleotides in mRNA that specify particular amino acids during protein synthesis. Each codon corresponds to a specific amino acid, as defined by the genetic code. Understanding this relationship is crucial for interpreting the results of the triplet binding technique, as it explains why only the amino acid that matches the presented codon will bind and remain on the filter.

Radioactive Labeling

Radioactive labeling involves attaching a radioactive isotope to a molecule, allowing for its detection and tracking in biological experiments. In the context of the triplet binding technique, the amino acid is labeled to visualize its binding to the codon. This method provides a clear indication of which amino acid corresponds to the codon, as only the labeled amino acid will remain on the filter, facilitating the study of translation fidelity.