Chapter 13, Problem 18
Many transcriptional activators are proteins with a DNA-binding domain (DBD) and an activation domain (AD). Explain how each domain contributes to transcriptional initiation. Would you expect repressors to also have each of these domains?
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Define the process of transcription. Where does this process fit into the central dogma of molecular biology (DNA makes RNA makes protein)?
Explain the features of the Initiator (Inr) elements, BREs, DPEs, and MTEs of focused promoters.
What observations suggested the existence of mRNA?
Describe the structure of RNA polymerase in bacteria. What is the core enzyme? What is the role of the σ subunit?
How do the ENCODE data vastly help determine which enhancers regulate which genes?
Write a paragraph describing the abbreviated chemical reactions that summarize RNA polymerase-directed transcription.