Chapter 13, Problem 13
A short RNA molecule was isolated that demonstrated a hyperchromic shift (see Chapter 10), indicating secondary structure. Its sequence was determined to be
5'-AGGCGCCGACUCUACU-3'
What DNA sequence would give rise to this RNA molecule through transcription?
Video transcript
In 1998, future Nobel laureates Andrew Fire and Craig Mello, and colleagues, published an article in Nature entitled, 'Potent and Specific Genetic Interference by Double-Stranded RNA in Caenorhabditis elegans.' Explain how RNAi is both 'potent and specific.'
Present an overview of RNA interference (RNAi). How does the silencing process begin, and what major components participate?
A short RNA molecule was isolated that demonstrated a hyperchromic shift (see Chapter 10), indicating secondary structure. Its sequence was determined to be
5'-AGGCGCCGACUCUACU-3'
Propose a two-dimensional model for this molecule.
RNAi may be directed by small interfering RNAs (siRNAs) or microRNAs (miRNAs); how are these similar, and how are they different?
Compare the control of gene regulation in eukaryotes and bacteria at the level of initiation of transcription. How do the regulatory mechanisms work? What are the similarities and differences in these two types of organisms in terms of the specific components of the regulatory mechanisms?
miRNAs target endogenous mRNAs in a sequence-specific manner. Explain, conceptually, how one might identify potential mRNA targets for a given miRNA if you only know the sequence of the miRNA and the sequence of all mRNAs in a cell or tissue of interest.