For the sequences given in the following list, indicate ...

Question

For the sequences given in the following list, indicate whether DNA replication, transcription, pre-mRNA processing, or translation will be most immediately affected by deletion of the sequence. As precisely as you can, specify what step of the process is directly affected by the deletion.

a. start codon
b. TATA box
c. 5' splice site
d. ori sequence
e. -10 consensus sequence
f. Shine–Dalgarno sequence
g. 5' cap
h. termination sequence

Accepted Answer

Hi, everyone. Let's take a look at this practice problem together. What is the position of the Shine Del Garno sequence in an M R N A? The answer options are a upstream of the start codon B downstream of the start codon C between the start and stop codons and D downstream of the stop codon. Now recall that the Shine Del Garno sequence is for pro Caros and it's for translation initiation. The sin is six nucleotides long A G G A G G and this is different than in New Caros. Now on the screen, I have a drawing of two M R N A strands, one for you Caros and one for pro Caros. Now in eukaryotes, the ribosome which is represented by a blue circle attaches to the five prime end of the M R N A translation then continues to the three prime end. However, in prokaryotes, the ribosome attaches to where the Shine Delno sequence is and that sequence is A G G A G G in prokaryotes. This brown circle is the ribosome attaching to the Shine Del Garno sequence. Now, once it attaches translation does occur in the five prime to three prime direction. That means that the shine del Garno sequence should be upstream of the star coat on. So the start code on could be downstream closer to the three prime end. If the sequence was downstream of the start codon, in any way, translation for certain genes could be skipped altogether. That means the correct answer is option a upstream of the start codon. All right, everyone. I hope you found this helpful and I'll see you soon for the next practice problem.

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