18. Molecular Genetic Tools
Genetic Cloning
Problem 19a
Textbook Question
Textbook QuestionIn Genetic Analysis 14.1 we designed a screen to identify conditional mutants of S. cerevisiae in which the secretory system was defective. Suppose we were successful in identifying 12 mutants. Based on your knowledge of the genetic tools for studying baker's yeast, how would you clone the genes that are mutated in your respective yeast strains? What is an approach to cloning the human orthologs (see Genetic Analysis 14.2 for definition) of the yeast genes?
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