18. Molecular Genetic Tools
Genetic Cloning
2:28 minutes
Problem 13
Textbook Question
Textbook QuestionIt is often desirable to insert cDNAs into a cloning vector in such a way that all the cDNA clones will have the same orientation with respect to the sequences of the plasmid. This is referred to as directional cloning. Outline how you would directionally clone a cDNA library in the plasmid vector pUC18.
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