Table of contents
- 1. Introduction to Genetics51m
- 2. Mendel's Laws of Inheritance3h 37m
- 3. Extensions to Mendelian Inheritance2h 41m
- 4. Genetic Mapping and Linkage2h 28m
- 5. Genetics of Bacteria and Viruses1h 21m
- 6. Chromosomal Variation1h 48m
- 7. DNA and Chromosome Structure56m
- 8. DNA Replication1h 10m
- 9. Mitosis and Meiosis1h 34m
- 10. Transcription1h 0m
- 11. Translation58m
- 12. Gene Regulation in Prokaryotes1h 19m
- 13. Gene Regulation in Eukaryotes44m
- 14. Genetic Control of Development44m
- 15. Genomes and Genomics1h 50m
- 16. Transposable Elements47m
- 17. Mutation, Repair, and Recombination1h 6m
- 18. Molecular Genetic Tools19m
- 19. Cancer Genetics29m
- 20. Quantitative Genetics1h 26m
- 21. Population Genetics50m
- 22. Evolutionary Genetics29m
11. Translation
The Genetic Code
3:35 minutes
Problem 30b
Textbook Question
Textbook QuestionA DNA sequence encoding a five-amino acid polypeptide is given below.
...ACGGCAAGATCCCACCCTAATCAGACCGTACCATTCACCTCCT...
...TGCCGTTCTAGGGTGGGATTAGTCTGGCATGGTAAGTGGAGGA...
Assuming the sequence above is a bacterial gene, identify the region encoding the Shine–Dalgarno sequence.
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Key Concepts
Here are the essential concepts you must grasp in order to answer the question correctly.
Shine-Dalgarno Sequence
The Shine-Dalgarno sequence is a ribosomal binding site in bacterial mRNA, crucial for initiating translation. It is typically located upstream of the start codon and is complementary to a sequence in the 16S rRNA of the ribosome, facilitating the assembly of the ribosome on the mRNA. This sequence ensures that the ribosome correctly identifies the start site for protein synthesis.
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Sequencing Difficulties
Translation Initiation
Translation initiation is the first step in protein synthesis, where the ribosome assembles around the mRNA. In bacteria, this process begins when the ribosome binds to the Shine-Dalgarno sequence, positioning the start codon in the ribosomal P site. This step is essential for the accurate translation of the genetic code into a polypeptide chain.
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Translation initiation
Bacterial Gene Structure
Bacterial genes are typically organized in operons and consist of coding sequences (exons) and regulatory elements. The coding region is flanked by promoter and terminator sequences, with the Shine-Dalgarno sequence located near the 5' end of the mRNA. Understanding this structure is vital for identifying functional elements like the Shine-Dalgarno sequence within a given DNA or RNA sequence.
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