5. Genetics of Bacteria and Viruses
Bacterial Conjugation
Problem 19c
Textbook Question
Textbook QuestionAn Hfr strain with the genotype cys⁺ lue⁺ met⁺ strˢ is mated with an strain carrying the genotype cys⁻ lue⁻ met⁻ strᴿ. In an interrupted mating experiment, small samples of the conjugating bacteria are withdrawn every 3 minutes for 30 minutes. The withdrawn cells are shaken vigorously to stop conjugation and then placed on three different selection media, composed as follows:
Medium 1: Minimal medium plus leucine, methionine, and streptomycin
Medium 2: Minimal medium plus cysteine, methionine, and streptomycin
Medium 3: Minimal medium plus cysteine, leucine, and streptomycin
What is the purpose of adding streptomycin to each selection medium?
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