So it's also important to note that the theoretical maximal reaction velocity or the V_max is actually directly impacted; the greater the total enzyme concentration, the greater the V_max will be. Before we get to our example down below, I want to point out that we should have already known this from our previous lesson video on the rate law. Recall that the V_max rate law is expressed right here. We covered this in our last lesson video, and we can clearly see that the total enzyme concentration will directly impact the V_max of the reaction; the greater this total enzyme concentration is, the greater the V_max will be.

If we take a look at our example down below to analyze the graph and get a more visual understanding of what this looks like, notice here in this graph that we have an enzyme kinetics plot where we have the initial reaction rates or the V₀ on the y-axis and the substrate concentration on the x-axis. We see two different curves here: a red curve and a green curve. This green curve has double the total concentration of enzymes compared to the red curve. Because it has double the total concentration, the theoretical maximum reaction velocity or the V_max is also doubled since the total enzyme concentration is also doubled.

We can see that by increasing the total enzyme concentration, this will also increase not only the initial reaction velocity but also the maximal reaction velocity, V_max. This is important to note as we move forward in our course when we're trying to compare the V_max of different enzyme-catalyzed reactions fairly. That concludes our lesson, and I'll see you guys in our next video.