Skip to main content
Ch. 3 - Observing Microorganisms Through a Microscope
Chapter 3, Problem 3.4a

Calculate the total magnification of the nucleus of a cell being observed through a compound light microscope with a 10x ocular lens and an oil immersion lens.

Verified step by step guidance
1
Identify the magnification power of the ocular lens, which is given as 10x.
Identify the magnification power of the oil immersion lens, which is typically 100x.
Understand that the total magnification in a compound light microscope is the product of the magnification of the ocular lens and the objective lens.
Multiply the magnification of the ocular lens (10x) by the magnification of the oil immersion lens (100x) to find the total magnification.
Express the total magnification as the product of the two magnifications, without calculating the final numerical value.

Verified Solution

Video duration:
2m
This video solution was recommended by our tutors as helpful for the problem above.
Was this helpful?

Key Concepts

Here are the essential concepts you must grasp in order to answer the question correctly.

Total Magnification

Total magnification in microscopy is the product of the magnification of the ocular lens and the magnification of the objective lens. For example, if the ocular lens has a magnification of 10x and the objective lens is an oil immersion lens typically magnifying 100x, the total magnification would be 10x multiplied by 100x, resulting in 1000x.
Recommended video:
Guided course
07:29
Magnification, Resolution, & Contrast

Ocular Lens

The ocular lens, or eyepiece, is the lens through which a microscope user looks to view the specimen. It usually has a fixed magnification, commonly 10x, and is essential for determining the total magnification when combined with the objective lens. Understanding its role is crucial for calculating how much larger the specimen appears compared to its actual size.
Recommended video:
Guided course
06:15
Components & Magnification of the Compound Light Microscope

Oil Immersion Lens

An oil immersion lens is a type of objective lens used in microscopy that requires a special immersion oil to enhance resolution and clarity. Typically, it has a high magnification, often 100x, and is designed to minimize light refraction, allowing for clearer images of small specimens. This lens is particularly important for observing cellular structures in detail.
Recommended video:
Guided course
06:15
Components & Magnification of the Compound Light Microscope
Related Practice
Textbook Question

Which type of microscope would be best to use to observe each of the following?

a. a stained bacterial smear

b. unstained bacterial cells: the cells are small, and no detail is needed

c. unstained live tissue when it is desirable to see some intracellular detail

d. a sample that emits light when illuminated with ultraviolet light

e. intracellular detail of a cell that is 1μm long

f. unstained live cells in which intracellular structures are shown in color

169
views
Textbook Question

Three-dimensional images of live cells can be produced with

a. darkfield microscopy.

b. fluorescence microscopy.

c. transmission electron microscopy.

d. confocal microscopy.

e. phase-contrast microscopy.

127
views
Textbook Question

Carbolfuchsin can be used as a simple stain and a negative stain. As a simple stain, the pH is

a. 2.

b. higher than the negative stain.

c. lower than the negative stain.

d. the same as the negative stain.

236
views
Textbook Question

Looking at the cell of a photosynthetic microorganism, you observe the chloroplasts are green in brightfield microscopy and red in fluorescence microscopy. You conclude:

a. chlorophyll is fluorescent.

b. the magnification has distorted the image.

c. you’re not looking at the same structure in both microscopes.

d. the stain masked the green color.

e. none of the above

118
views
Textbook Question

The maximum magnification of a compound microscope is (a) ________; that of an electron microscope, (b) ________. The maximum resolution of a compound microscope is (c) ________; that of an electron microscope, (d) ________. One advantage of a scanning electron microscope over a transmission electron microscope is (e) ________.

130
views
Textbook Question

Which of the following is not a functionally analogous pair of stains?

a. nigrosin and malachite green

b. crystal violet and carbolfuchsin

c. safranin and methylene blue

d. ethanol-acetone and acid-alcohol

e. All of the above pairs are functionally analogous.

118
views