Assume you stain Clostridium by applying a basic stain, carbolfuchsin, with heat, decolorizing with acid-alcohol, and counterstaining with an acidic stain, nigrosin. Through the microscope, the endospores are 1, and the cells are stained 2.
a. 1—red; 2—black
b. 1—black; 2—colorless
c. 1—colorless; 2—black
d. 1—red; 2—colorless
e. 1—black; 2—red

Question

Assume you stain Clostridium by applying a basic stain, carbolfuchsin, with heat, decolorizing with acid-alcohol, and counterstaining with an acidic stain, nigrosin. Through the microscope, the endospores are 1, and the cells are stained 2.
a. 1—red; 2—black
b. 1—black; 2—colorless
c. 1—colorless; 2—black
d. 1—red; 2—colorless
e. 1—black; 2—red

a. 1—red; 2—black

b. 1—black; 2—colorless

c. 1—colorless; 2—black

d. 1—red; 2—colorless

e. 1—black; 2—red

Accepted Answer

Hi, everyone. Let's take a look at this practice problem together. C tetany is a spore forming slender anaerobic rod infamous for causing tetanus, which of the following is used to identify C tetany. The answer options are a gram staining B blood agar media, C, both A and B and option D. None of the above recall that there are different laboratory techniques that can be used to identify organisms, both gram staining and blood agar media are techniques that can be used for identification. So let's review them. Option. A recall that gram staining uses crystal violet dye to stain and classify bacteria as either gram positive or gram negative. And that is based on the bacteria cell wall composition. This method requires the use of a microscope. Option B blood agar media recall is an enriched media in specific because it contains red blood cells. So it's useful for differentiate differentiating bacteria that can break down red blood cells. So, bacteria that are hemolytic, it is also used to grow microbes that have complex nutritional requirements and do not grow easily on other mediums. Now, the bacteria in question, Clostridium tetany. It's commonly found in soil and the G I tracts of animals, it causes tetanus when the spores enter the human body, which is often by wounds. It can be identified by both gram staining and blood agar media. C techni is gram positive and on blood agar media, it grows as a swarm. And swar means that it doesn't form distinct colonies. So both options A and B are correct. So the correct answer is option C, both A and B. All right. Everyone. I hope you found this helpful and I'll see you soon for the next practice problem.

Verified Solution

Key Concepts

Endospore Staining

Counterstaining

Acid-Alcohol Decolorization