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Ch. 13 - Viruses, Viroids, and Prions
Chapter 10, Problem 13.1a

Place the following in the most likely order for biosynthesis of a bacteriophage: (1) phage lysozyme; (2) mRNA; (3) DNA; (4) viral proteins; (5) DNA polymerase.
a. 5, 4, 3, 2, 1
b. 1, 2, 3, 4, 5
c. 5, 3, 4, 2, 1
d. 3, 5, 2, 4, 1
e. 2, 5, 3, 4, 1

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1
Identify the initial step in the biosynthesis of a bacteriophage, which typically involves the synthesis of mRNA from the viral DNA.
Determine the role of DNA polymerase, which is responsible for replicating the viral DNA.
Consider the synthesis of viral proteins, which are translated from the mRNA.
Recognize the function of phage lysozyme, which is involved in the later stages of the bacteriophage life cycle, aiding in the release of new virions.
Arrange the steps in a logical sequence based on the typical order of events in bacteriophage biosynthesis: mRNA synthesis, DNA replication, protein synthesis, and finally, the action of lysozyme.

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Key Concepts

Here are the essential concepts you must grasp in order to answer the question correctly.

Bacteriophage Life Cycle

Bacteriophages, or phages, are viruses that infect bacteria. Their life cycle typically involves attachment to a host cell, injection of viral DNA, and subsequent replication of viral components. Understanding the sequence of events in the life cycle is crucial for determining the order of biosynthesis processes, including the production of mRNA and proteins.
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Viral Replication and Transcription

During viral replication, the viral DNA is transcribed into mRNA, which serves as a template for synthesizing viral proteins. This process is facilitated by viral enzymes, such as DNA polymerase, which is essential for replicating the viral genome. Recognizing the roles of these components helps in understanding the correct sequence of biosynthesis.
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Role of Phage Lysozyme

Phage lysozyme is an enzyme produced by bacteriophages that helps in breaking down the bacterial cell wall, facilitating the release of new virions. It is typically one of the last steps in the phage life cycle, occurring after the viral components have been synthesized and assembled. Knowing when lysozyme acts in the process is key to ordering the steps of biosynthesis correctly.
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