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Ch. 8 - Microbial Metabolism
Chapter 8, Problem 8.13a

The photo shows an organism growing in glucose fermentation broth. What can you conclude about this specimen with regard to its ability (or lack thereof) to ferment the sugar sucrose? Explain your answer.
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Step 1: Understand the context of the problem. The organism is growing in a glucose fermentation broth, which indicates it can ferment glucose.
Step 2: Consider the metabolic pathways involved. Fermentation of sugars like glucose and sucrose involves different enzymes and pathways.
Step 3: Recognize that glucose and sucrose are different sugars. Glucose is a monosaccharide, while sucrose is a disaccharide composed of glucose and fructose.
Step 4: Determine if the ability to ferment glucose implies the ability to ferment sucrose. The organism must have the enzyme sucrase to break down sucrose into glucose and fructose before fermentation.
Step 5: Conclude that without additional information or tests, you cannot definitively conclude the organism's ability to ferment sucrose based solely on its ability to ferment glucose.

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Key Concepts

Here are the essential concepts you must grasp in order to answer the question correctly.

Fermentation

Fermentation is a metabolic process that converts sugars into acids, gases, or alcohol in the absence of oxygen. Microorganisms, such as bacteria and yeast, utilize fermentation to generate energy. The ability to ferment specific sugars, like glucose or sucrose, can be determined by observing the production of gas or acid in a fermentation broth.
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Sugar Specificity

Different microorganisms have varying abilities to ferment specific sugars due to the presence of specific enzymes. For instance, while some organisms can ferment glucose, they may lack the necessary enzymes to ferment sucrose. Understanding the metabolic pathways of the organism in question is crucial for predicting its fermentation capabilities.
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Broth Indicators

Fermentation broths often contain pH indicators that change color in response to acid production, signaling fermentation activity. If the organism in the image produces acid from glucose fermentation, it suggests metabolic activity, but it does not directly indicate sucrose fermentation ability. Observing the broth's characteristics can provide insights into the organism's fermentation profile.
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Related Practice
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_______________________ is a process that uses energy from nutrients to fuel an electron transport chain to make ATP.

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The _______________________ test detects if an organism can convert hydrogen peroxide to water and oxygen. The test result is usually _______________________ in anaerobic microbes and usually _______________________ in aerobic microbes. You know the test is positive by _______________________.

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Textbook Question

Use this pathway schematic to answer questions a through d.

a. Which enzyme carries out a redox reaction? In this reaction, what is being reduced and what is being oxidized?

b. Which enzyme would be the most likely to be regulated by feedback inhibition?

c. What is the end product of this pathway?

d. Which enzyme carries out substrate-level phosphorylation?

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Textbook Question

Match the term to the statement. (Some terms will be used more than once.)

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Textbook Question

Rank the following from the most ATP that could be made to the least ATP that could be made: (NCLEX/HESI/TEAS)

a. 1 glucose molecule processed via a fermentation pathway (consider that glycolysis is the first stage of the process)

b. A lipid made of glycerol and three 10-carbon fatty acid chains entering cellular respiration

c. 1 glucose molecule entering the Entner–Doudoroff pathway

d. 1 glucose molecule entering cellular respiration

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